啮齿动物脑垂体中间叶中促肾上腺皮质激素相关肽：通过高效液相色谱法和放射免疫分析法进行表征

Corticotrophin-related peptides in the intermediate lobe of the rodent pituitary gland: characterization by high performance liquid chromatography and radioimmunoassay.

作者信息

Al-Noaemi M C, Biggins J A, Edwardson J A, McDermott J R, Smith A I

出版信息

Regul Pept. 1982 May;3(5-6):351-9. doi: 10.1016/0167-0115(82)90058-1.

Abstract

High performance liquid chromatography (HPLC) and radioimmunoassay have been used to characterize corticotrophin-related peptides in extracts of the intermediate lobe of the rat and mouse pituitary gland. Multiple peaks have been observed, which resemble corticotrophin-like intermediate lobe peptide (CLIP) in that they cross-react with antisera raised against the COOH-terminal region of corticotrophin (ACTH) but not against NH2-terminal directed antisera. These CLIP-like peptides were released from the incubated neurointermediate lobe and their secretion was inhibited in the presence of dopamine. Heterogeneity of peptide species was also observed with antisera raised against alpha-MSH. Multiple peaks of CLIP and alpha-MSH-like material were identified in pituitary extracts from the mouse and levels were elevated in the genetically obese (ob/ob) animal. The nature and possible functions of multiple forms of intermediate lobe peptides are discussed.

摘要

高效液相色谱法（HPLC）和放射免疫分析法已被用于鉴定大鼠和小鼠脑垂体中间叶提取物中与促肾上腺皮质激素相关的肽。观察到多个峰，它们类似于促肾上腺皮质激素样中间叶肽（CLIP），因为它们与针对促肾上腺皮质激素（ACTH）COOH末端区域产生的抗血清发生交叉反应，但不与针对NH2末端定向抗血清发生交叉反应。这些CLIP样肽从孵育的神经中间叶释放出来，并且在多巴胺存在下其分泌受到抑制。用针对α-MSH产生的抗血清也观察到肽种类的异质性。在小鼠的垂体提取物中鉴定出多个CLIP和α-MSH样物质峰，并且在遗传性肥胖（ob/ob）动物中水平升高。讨论了中间叶肽多种形式的性质和可能的功能。