Frixen U, Von der Helm K, Friis R, Wille W, Willecke K
J Gen Virol. 1982 Jul;61 (Pt l):83-6. doi: 10.1099/0022-1317-61-1-83.
Purified virus proteins from transformation-defective (td) mutants of Rous sarcoma virus PrA or PrB were trapped in human erythrocyte ghosts which, after resealing, were fusion-injected into hamster RBH cells or rat TWERC cells. These cell lines are non-productively transformed by subgroup C Rous sarcoma virus. After fusion injection the hamster RBH cells released transforming subgroup C Rous sarcoma virus. No infectious virus could be rescued from rat TWERC cells. Since previous experiments have shown that fusion injection of the purified Rous sarcoma virus protein p15 into hamster RBH cells caused cleavage of the precursor protein pr76 to form the virus group-specific antigen (gag) but did not induce infectious virus, we conclude that in addition to p15 other virus proteins are required to induce virus rescue in hamster RBH cells.
从劳斯肉瘤病毒PrA或PrB的转化缺陷（td）突变体中纯化的病毒蛋白被捕获在人红细胞血影中，重新封闭后，融合注射到仓鼠RBH细胞或大鼠TWERC细胞中。这些细胞系被C亚群劳斯肉瘤病毒非生产性转化。融合注射后，仓鼠RBH细胞释放出转化性C亚群劳斯肉瘤病毒。从大鼠TWERC细胞中未能拯救出感染性病毒。由于先前的实验表明，将纯化的劳斯肉瘤病毒蛋白p15融合注射到仓鼠RBH细胞中会导致前体蛋白pr76裂解形成病毒群特异性抗原（gag），但不会诱导感染性病毒，我们得出结论，除了p15之外，还需要其他病毒蛋白来诱导仓鼠RBH细胞中的病毒拯救。
