血管活性肠肽对青蛙神经肌肉传递的影响。
The effects of vasoactive intestinal peptide on neuromuscular transmission in the frog.
作者信息
Gold M R
出版信息
J Physiol. 1982 Jun;327:325-35. doi: 10.1113/jphysiol.1982.sp014234.
Abstract
- The effects of vasoactive intestinal peptide (VIP) on cholinergic transmission were studied at the neuromuscular junction of the frog.2. Bath application of VIP (10(-8)-10(-7) M) produced a dose-dependent increase in quantal content of the end-plate potential (e.p.p.), while having no effect on quantal size or on resting membrane potential of the muscle fibres.3. The increases in e.p.p. amplitude observed at end-plates blocked with Mg(2+) or curare were comparable.4. VIP increased miniature end-plate potential frequency when the nerve terminal was depolarized with K(+). This effect was abolished by removing Ca(2+) from the bathing solution.5. Ionophoretic application of VIP to the end-plate also increased quantal content, while having no effect on quantal size or on the input resistance and resting membrane potential of the fibres.6. Some possible mechanisms for the action of VIP are discussed, and it is proposed that it may act on some step involved in depolarization-secretion coupling.
摘要
研究了血管活性肠肽（VIP）对青蛙神经肌肉接头处胆碱能传递的影响。
浴槽中加入VIP（10⁻⁸ - 10⁻⁷ M）可使终板电位（e.p.p.）的量子含量呈剂量依赖性增加，而对量子大小或肌纤维的静息膜电位无影响。
在被Mg²⁺或箭毒阻断的终板处观察到的e.p.p.幅度增加相当。
当用K⁺使神经末梢去极化时，VIP增加了微小终板电位频率。从浴槽溶液中去除Ca²⁺可消除此效应。
向终板离子电泳施加VIP也增加了量子含量，而对量子大小或纤维的输入电阻和静息膜电位无影响。
讨论了VIP作用的一些可能机制，并提出它可能作用于去极化-分泌偶联所涉及的某些步骤。
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