骨骼肌肌动蛋白对牛和大鼠腮腺脱氧核糖核酸酶I的抑制作用。一项生物化学和免疫细胞化学研究。

Mannherz H G, Kreuder V, Koch J, Dieckhoff J, Drenckhahn D

Biochem J. 1982 Nov 1;207(2):305-13. doi: 10.1042/bj2070305.

Rat and bovine parotid gland and pancreas contain deoxyribonuclease I (DNAase I) activities in different amounts. The DNAase I activity in tissue homogenates of bovine and rat parotid gland can be inhibited by addition of monomeric actin, as with the enzyme of bovine pancreas. The isolated DNAase I species from bovine and rat parotid gland differ in their molecular weights and also in their affinities for monomeric actin, being lowest for rat parotid DNAase I (5 X 10(6)M(-1). Antibodies raised against rat and bovine parotid and bovine pancreatic DNAase I can be used to study the subcellular localization of DNAase I in these tissues by indirect immunofluorescence. DNAase I was found to be confined solely to the secretory granules of the tissue from which it was isolated.

大鼠和牛的腮腺及胰腺中含有不同量的脱氧核糖核酸酶I（DNAase I）活性。与牛胰腺的酶一样，牛和大鼠腮腺组织匀浆中的DNAase I活性可通过添加单体肌动蛋白来抑制。从牛和大鼠腮腺中分离出的DNAase I种类在分子量以及对单体肌动蛋白的亲和力方面存在差异，大鼠腮腺DNAase I的亲和力最低（5×10⁶M⁻¹）。针对大鼠和牛的腮腺以及牛胰腺DNAase I产生的抗体可用于通过间接免疫荧光研究这些组织中DNAase I的亚细胞定位。结果发现，DNAase I仅局限于其分离来源组织的分泌颗粒中。