鸡胚核糖核酸酶H对与聚（dT）模板互补的共价连接的聚（A）-聚（dA）的作用。
Effect of ribonuclease H from chick embryo on the covalent-linked poly(A)--poly(dA) complementary to poly(dT) template.
作者信息
Sawai Y, Kitahara N, Tsukada K
出版信息
FEBS Lett. 1982 Dec 13;150(1):228-32. doi: 10.1016/0014-5793(82)81340-9.
In vitro poly(dA) synthesis on poly(dT) template can be initiated by poly(A) primer. Poly(A) chains are covalently extended by DNA polymerase. The reaction product consists of poly(dA) chain with poly(A) at their 5'-ends, hydrogen bonded to the template poly(dT). The primer poly(A) is linked to the product poly(dA) via a 3':5'-phosphodiester bond, and can be specifically removed by ribonuclease H from chick embryos, leaving a 5'-phosphate end of poly(dA). Poly- or oligoriboadenylate longer than the (pA)5 could serve as a priming activity to synthesize poly(A) covalently linked to poly(dA).
在聚（dT）模板上进行的体外聚（dA）合成可由聚（A）引物引发。聚（A）链通过DNA聚合酶共价延伸。反应产物由5'-端带有聚（A）的聚（dA）链组成，通过氢键与模板聚（dT）结合。引物聚（A）通过3':5'-磷酸二酯键与产物聚（dA）相连，并且可以被来自鸡胚的核糖核酸酶H特异性去除，从而留下聚（dA）的5'-磷酸末端。长度超过（pA）5的多聚或寡聚核糖腺苷酸可作为引发活性物质来合成与聚（dA）共价连接的聚（A）。
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