Nemoto N, Takayama S
Gan. 1982 Dec;73(6):887-92.
The effects of hemin on the metabolism of benzo[a]pyrene (BP) in rat liver microsomes were studied. Hemin inhibited the activity of aryl hydrocarbon hydroxylase and the metabolic activation of BP to forms able to bind to DNA. Analyses by high performance liquid chromatography of metabolites of BP revealed that hemin reduced their production and changed their relative amounts. The decrease in the amount of BP-7,8-dihydrodiol was greater than that in the amount of phenols. The elution profile of BP-metabolite-bound deoxyribonucleosides on Sephadex LH-20 column chromatography indicated that the formation of adducts with BP-7,8-dihydrodiol-9,10-oxide was decreased by hemin more than the formation of those with phenol derivatives. The present results suggest that endogenous components that are not enzymes are important modifiers of the biological activities of chemical carcinogens.
研究了血红素对大鼠肝微粒体中苯并[a]芘（BP）代谢的影响。血红素抑制芳烃羟化酶的活性以及BP代谢活化为能够与DNA结合的形式。通过高效液相色谱法对BP代谢产物的分析表明，血红素减少了它们的产生并改变了它们的相对含量。BP - 7,8 - 二氢二醇量的减少大于酚类量的减少。在Sephadex LH - 20柱色谱上BP代谢物结合的脱氧核糖核苷的洗脱图谱表明，与BP - 7,8 - 二氢二醇 - 9,10 - 环氧化物形成加合物的量比与酚类衍生物形成加合物的量更多地被血红素减少。目前的结果表明，非酶的内源性成分是化学致癌物生物活性的重要调节剂。
