Kuramoto A, Otani S, Matsui I, Morisawa S
FEBS Lett. 1983 Jan 24;151(2):233-6. doi: 10.1016/0014-5793(83)80076-3.
Treatment of guinea pig lymphocytes with Clostridium perfringens phospholipase C but not with Naja naja snake venom phospholipase A2 increased ornithine decarboxylase activity. The increase in ornithine decarboxylase activity was suppressed by actinomycin D or cycloheximide, suggesting that de novo syntheses of RNA and protein are necessary for the increase in the enzyme activity. These results suggest that the activation of phospholipase C rather than that of phospholipase A2 is responsible for induction of ornithine decarboxylase during lymphocyte transformation.
用产气荚膜梭菌磷脂酶C而非眼镜蛇蛇毒磷脂酶A2处理豚鼠淋巴细胞，可增加鸟氨酸脱羧酶的活性。放线菌素D或环己酰亚胺可抑制鸟氨酸脱羧酶活性的增加，这表明RNA和蛋白质的从头合成对于该酶活性的增加是必需的。这些结果表明，在淋巴细胞转化过程中，磷脂酶C而非磷脂酶A2的激活是诱导鸟氨酸脱羧酶的原因。
