Christiansen G, Christiansen C, Zeuthen J
J Cancer Res Clin Oncol. 1983;105(1):13-9. doi: 10.1007/BF00391825.
Human lymphocytes and lymphoid cell lines were analyzed for the presence of complex forms of mitochondrial DNA (mtDNA) by electron microscopy. A high frequency (9%-14.5%) of catenated dimers, circular dimers, or oligomers were found in samples from Epstein-Barr-virus-(EBV) transformed lymphoblastoid cell lines. These complex forms of mtDNA were present in much lower frequencies in lymphocytes isolated from donor blood (1.3%-4.6%). Similar low frequencies were found with primary fibroblasts (1.1%) or freshly isolated monkey liver cells (2.1%). Samples from cultures of Burkitt lymphoma (BL) cell lines of EBV-positive or -negative origin contained intermediate (5%-7%) frequencies of complex forms of mtDNA.
通过电子显微镜分析人淋巴细胞和淋巴样细胞系，以检测线粒体DNA（mtDNA）复杂形式的存在情况。在来自爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞系的样本中，发现了高频（9%-14.5%）的连环二聚体、环状二聚体或寡聚体。这些mtDNA的复杂形式在从供血者血液中分离出的淋巴细胞中出现的频率要低得多（1.3%-4.6%）。在原代成纤维细胞（1.1%）或新鲜分离的猴肝细胞（2.1%）中也发现了类似的低频率。来自EBV阳性或阴性来源的伯基特淋巴瘤（BL）细胞系培养物的样本中，mtDNA复杂形式的频率处于中等水平（5%-7%）。
