James J E, James D M, Martin P A, Reed D E, Davis D L
J Am Vet Med Assoc. 1983 Sep 1;183(5):525-8.
Embryo transfer was used to conserve genetic material from 2 swine herds seropositive for pseudorabies virus (PRV). Embryos (n = 805) were recovered from 38 PRV-seropositive Duroc sows in Iowa and, after 4 to 10 hours' culture and shipment to Illinois, were transferred to 34 recipients from a herd seronegative for PRV. All recipients remained seronegative for PRV, and 22 of the recipients farrowed 208 pigs (189 alive) that also were seronegative for PRV. There was no evidence of PRV in the embryo recovery medium or in the uterine and oviductal cells recovered with the embryos. Transfer of morulae resulted in higher (P less than 0.02) farrowing rates than did transfer of 4- to 8-cell embryos, but litter size was not affected.
采用胚胎移植技术保存来自2个伪狂犬病病毒(PRV)血清学阳性猪群的遗传物质。从艾奥瓦州38头PRV血清学阳性的杜洛克母猪中采集胚胎(n = 805),在培养4至10小时并运往伊利诺伊州后,将其移植到来自一个PRV血清学阴性猪群的34头受体母猪体内。所有受体母猪PRV血清学检测仍为阴性,其中22头受体母猪产下208头仔猪(189头存活),这些仔猪PRV血清学检测也为阴性。在胚胎回收培养液以及与胚胎一同回收的子宫和输卵管细胞中均未发现PRV迹象。桑葚胚移植的产仔率高于4至8细胞胚胎移植(P < 0.02),但窝产仔数不受影响。