鼠伤寒沙门氏菌肽酶T的过量产生。
Overproduction of Salmonella typhimurium peptidase T.
作者信息
Strauch K L, Carter T H, Miller C G
出版信息
J Bacteriol. 1983 Nov;156(2):743-51. doi: 10.1128/jb.156.2.743-751.1983.
Abstract
Pseudorevertants able to use L-leucyl-L-leucyl-L-leucine as a leucine source have been isolated from a Salmonella typhimurium strain carrying stable (nonreverting) mutations in pepN, pepA, and pepB. These strains carry mutations at a locus pto (peptidase T overproducer) tightly linked to pepT that cause an elevated expression of the tripeptidase peptidase T. An F' episome carrying the pto and pepT loci has been constructed and used to show that the pto mutations are cis dominant. Expression of beta-galactosidase from a Mu d1(Apr lac) insertion in pepT is increased by pto mutations. The pto mutations, therefore, define a site affecting the transcription of pepT.
摘要
能够将L-亮氨酰-L-亮氨酰-L-亮氨酸用作亮氨酸来源的假回复突变体已从一株携带pepN、pepA和pepB稳定（非回复）突变的鼠伤寒沙门氏菌菌株中分离出来。这些菌株在与pepT紧密连锁的pto（肽酶T过度生产者）位点发生突变，导致三肽酶肽酶T的表达升高。构建了携带pto和pepT位点的F'附加体，并用于证明pto突变是顺式显性的。pto突变会增加来自pepT中Mu d1(Apr lac)插入的β-半乳糖苷酶的表达。因此，pto突变定义了一个影响pepT转录的位点。
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本文引用的文献
1
Hfr formation directed by tn10.
Genetics. 1979 Apr;91(4):639-55. doi: 10.1093/genetics/91.4.639.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
Genetic fine structure of the leucine operon in Salmonella.
Genetics. 1963 Mar;48(3):441-57. doi: 10.1093/genetics/48.3.441.
4
Abstracts of papers presented at the 1980 meetings of the Genetic Society of America. Boulder, Colorado August 18-20, 1980.
Genetics. 1980;94(4 Pt 2 Suppl):1-16.
5
Isolation and characterization Salmonella typhimurium mutants lacking a tripeptidase (peptidase T).
J Bacteriol. 1983 May;154(2):763-71. doi: 10.1128/jb.154.2.763-771.1983.
6
Dipeptidyl carboxypeptidase-deficient mutants of Salmonella typhimurium.
J Bacteriol. 1983 Mar;153(3):1252-8. doi: 10.1128/jb.153.3.1252-1258.1983.
7
Positive selection for loss of tetracycline resistance.
J Bacteriol. 1980 Aug;143(2):926-33. doi: 10.1128/jb.143.2.926-933.1980.
8
A new method of peptidase assay and the separation of three leucylglycinases of renal tissues.
Arch Biochem Biophys. 1968 Nov;128(2):397-405. doi: 10.1016/0003-9861(68)90046-5.
9
Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella.
Adv Genet. 1971;16:1-34. doi: 10.1016/s0065-2660(08)60352-1.
10
Evolution of a new gene substituting for the leuD gene of Salmonella typhimurium: origin and nature of supQ and newD mutations.
J Bacteriol. 1974 Dec;120(3):1176-85. doi: 10.1128/jb.120.3.1176-1185.1974.
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