Tognon M, Cassai E, Rotola A, Roizman B
Microbiologica. 1983 Jul;6(3):191-8.
Restriction endonuclease analyses of herpes simplex virus (HSV-1) DNAs revealed variability in the electrophoretic migration of specific Bam HI and Kpn I fragments. The variable regions of the genome map at the ends and near the ends of both L and S components, near the L-S junction region and in middle of the S component. The variability of the fragments was demonstrated by comparing the electrophoretic mobility patterns of DNAs from plaque-purified stocks originally derived from a common parental plaque-purified virus stock. Our analyses suggest that DNA sequences contained in the variable fragments can undergo accretion or deletion. So far the variability of the HSV-DNA molecules has been explained in terms of number of copies of terminally reiterated sequences (Wagner et al., 1978, Locker et al., 1979, Lonsdale et al., 1980, Davison et al., 1981). Our results may be explained by unequal crossing over, slippage, because of the presence of reiterated sequences, or by the presence of transposable elements.
对单纯疱疹病毒1型（HSV - 1）DNA进行的限制性内切酶分析显示，特定的Bam HI和Kpn I片段在电泳迁移方面存在变异性。基因组的可变区位于L和S组分的末端及靠近末端处、L - S连接区附近以及S组分的中部。通过比较最初源自共同亲代噬斑纯化病毒株的噬斑纯化病毒株DNA的电泳迁移模式，证实了片段的变异性。我们的分析表明，可变片段中包含的DNA序列可能会发生增加或缺失。到目前为止，HSV - DNA分子的变异性已根据末端重复序列的拷贝数来解释（瓦格纳等人，1978年；洛克等人，1979年；朗斯代尔等人，1980年；戴维森等人，1981年）。我们的结果可以用不等交换、由于重复序列的存在而导致的滑动或者转座元件的存在来解释。
