[百日咳博德特氏菌DNA在大肠杆菌细菌细胞系统中的克隆]
[Cloning of Bordetella pertussis DNA in an Escherichia coli bacterial cell system].
作者信息
Semina I E, Tarasov A P, Mileĭkovskaia M M, Kazennova E V, Tsvetkova N V
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1983 Aug(8):17-21.
PMID:6314709
Abstract
Hybrid plasmids containing B. pertussis DNA insertions have been constructed with the use of the vector plasmid pBR 322 and the Pst I fragments of B. pertussis DNA. Some properties of the hybrid plasmids are characterized. The possibility of the expression of B. pertussis genes in the protein-synthetizing cell-free system obtained from E. coli has been demonstrated.
摘要
利用载体质粒pBR 322和百日咳博德特氏菌DNA的Pst I片段构建了含有百日咳博德特氏菌DNA插入片段的杂种质粒。对杂种质粒的一些特性进行了表征。已经证明了百日咳博德特氏菌基因在从大肠杆菌获得的无细胞蛋白质合成系统中表达的可能性。
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