普萘洛尔和吲哚氰绿心安与大鼠肝脏、肺、肾脏和心脏的分离细胞、匀浆及质膜的结合。

Binding of propranolol and iodocyanopindolol to isolated cells, homogenates and plasma membranes of rat liver, lung, kidney and heart.

作者信息

Zini R, Gault I, Ledewyn S, D'Athis P, Tillement J P

出版信息

Biochem Pharmacol. 1983 Nov 15;32(22):3375-80. doi: 10.1016/0006-2952(83)90365-9.

Abstract

3H-(+/-) propranolol and 125I-(+/-) cyanopindolol have been used to characterize beta adrenoceptors of liver, lung, kidney and heart of rat. Two main binding parameters, KD and Bmax were measured using either cells, homogenates or plasma membranes of each organ (except heart). Results show that the most accurate determination of KD and Bmax involves: (1) a previous extraction of plasma membranes (2) the use of a ligand of a high affinity for beta adrenoceptors (3) a high specific radioactivity of this ligand. 125I-(+/-) cyanopindolol seems to be a better ligand than 3H(+/-) propranolol for such determinations.

摘要

3H-(+/-)普萘洛尔和125I-(+/-)氰吲哚洛尔已被用于表征大鼠肝脏、肺、肾脏和心脏的β肾上腺素能受体。使用每个器官（心脏除外）的细胞、匀浆或质膜测量了两个主要结合参数KD和Bmax。结果表明，KD和Bmax的最准确测定涉及：(1) 质膜的预先提取；(2) 使用对β肾上腺素能受体具有高亲和力的配体；(3) 该配体的高比放射性。对于此类测定，125I-(+/-)氰吲哚洛尔似乎是比3H(+/-)普萘洛尔更好的配体。