Bernal S D, Speak J A
Cancer Res. 1984 Jan;44(1):265-70.
Mouse myeloma cells were fused with spleen cells from BALB/c mice immunized with a cell line derived from human small cell carcinoma (SCC) of the lung. The cloned hybridoma SM1 produced antibody that was reactive with the surface membrane of SCC cell lines and SCC tumors but not with the membrane of several non-SCC cell lines and tumors. SM1 ascites fluid was used to screen for reactivity of the antibody with other human cancer cell lines, tumor tissues, and normal tissues. SM1 antibody was found to be unreactive with neuroblastoma, adrenal carcinoma, melanoma, and bronchial carcinoid. Reactivity was detected with some breast carcinoma cell lines but not with breast cancer tissue specimens. In the same individual, the antibody was reactive with SCC lung tumor and SCC metastatic to the liver but not with normal tissues, including bronchus, lung parenchyma, liver, kidney, and brain. Human erythrocytes and marrow cells were also unreactive. Since SM1 detects an antigen that is present in greatest amounts on the surface membrane of SCC of the lung, this antibody may be useful in tracing the lineage patterns of human lung cancers.
将小鼠骨髓瘤细胞与用源自人肺小细胞癌（SCC）的细胞系免疫的BALB/c小鼠的脾细胞进行融合。克隆的杂交瘤SM1产生的抗体可与SCC细胞系和SCC肿瘤的表面膜发生反应，但不与几种非SCC细胞系和肿瘤的膜发生反应。使用SM1腹水来筛选该抗体与其他人类癌细胞系、肿瘤组织和正常组织的反应性。发现SM1抗体与神经母细胞瘤、肾上腺癌、黑色素瘤和支气管类癌无反应。在一些乳腺癌细胞系中检测到反应性，但在乳腺癌组织标本中未检测到。在同一个体中，该抗体与肺SCC肿瘤和转移至肝脏的SCC反应，但不与正常组织反应，包括支气管、肺实质、肝脏、肾脏和脑。人红细胞和骨髓细胞也无反应。由于SM1检测到一种在肺SCC表面膜上含量最高的抗原，该抗体可能有助于追踪人类肺癌的谱系模式。
