肌肉中葡萄糖-6-磷酸酶放射化学测定法中的一个问题。
A problem in the radiochemical assay of glucose-6-phosphatase in muscle.
作者信息
Lackner R, Challiss R A, West D, Newsholme E A
出版信息
Biochem J. 1984 Mar 1;218(2):649-51. doi: 10.1042/bj2180649.
Abstract
A radiochemical assay for glucose-6-phosphatase, which depends on the absorption of radiolabelled substrate on to a nascent precipitate of BaSO4 and Zn(OH)2, can give rise to erroneously high activities. Adsorption on to a Fe(OH)3 precipitate does not expose the incubation medium to extreme alkaline conditions and provides a satisfactory and simple assay. This method demonstrates a very low activity of the enzyme in mammalian muscle, in contrast with previous findings.
摘要
一种用于检测葡萄糖-6-磷酸酶的放射化学分析方法，该方法依赖于放射性标记底物吸附到硫酸钡和氢氧化锌的新生沉淀上，可能会导致活性测定结果错误地偏高。吸附到氢氧化铁沉淀上不会使孵育介质暴露于极端碱性条件下，并且提供了一种令人满意且简单的分析方法。与之前的研究结果相反，该方法显示哺乳动物肌肉中该酶的活性非常低。
相似文献
1
A problem in the radiochemical assay of glucose-6-phosphatase in muscle.肌肉中葡萄糖-6-磷酸酶放射化学测定法中的一个问题。
Biochem J. 1984 Mar 1;218(2):649-51. doi: 10.1042/bj2180649.
2
Glucose and fructose-6-phosphatase cycling by rat hepatocytes at gluconeogenesis.大鼠肝细胞在糖异生过程中的葡萄糖与6-磷酸果糖磷酸酶循环
Acta Univ Carol Med (Praha). 1981;27(7-8):477-90.
3
Histochemical investigations on the activity of some phosphatases in skeletal muscles of mice under the influence of infection with Trichinella spiralis.
Folia Histochem Cytochem (Krakow). 1971;9(1):95-116.
4
5
[Phosphoprotein phosphatase of muscles of different animal species].
Ukr Biokhim Zh. 1971 Jul-Aug;43(4):459-62.
6
[Effect of 4-methyluracil on enzymes of glycogen and glucose-6-phosphate metabolism in liver].[4-甲基尿嘧啶对肝脏中糖原及6-磷酸葡萄糖代谢相关酶的影响]
Ukr Biokhim Zh. 1969;41(2):150-6.
7
Ultrastructural localization of several phosphatases with cerium.几种磷酸酶与铈的超微结构定位
J Histochem Cytochem. 1983 Oct;31(10):1197-208. doi: 10.1177/31.10.6309949.
8
Effect of continuous glucose infusion on the glucose-6-phosphatase activity of the liver in rats.持续输注葡萄糖对大鼠肝脏葡萄糖-6-磷酸酶活性的影响。
N Engl J Med. 1959 Aug 27;261:434-7. doi: 10.1056/NEJM195908272610905.
9
Histone II-A stimulates glucose-6-phosphatase and reveals mannose-6-phosphatase activities without permeabilization of liver microsomes.组蛋白II - A刺激葡萄糖-6-磷酸酶，并在不使肝微粒体通透的情况下显示出甘露糖-6-磷酸酶活性。
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):221-4. doi: 10.1042/bj3100221.
10
Maximum activities and properties of glucose 6-phosphatase in muscles from vertebrates and invertebrates.脊椎动物和无脊椎动物肌肉中葡萄糖-6-磷酸酶的最大活性和特性。
Biochem J. 1981 Sep 15;198(3):621-9. doi: 10.1042/bj1980621.
引用本文的文献
1
Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running.测量小鼠在急性跑步机跑步后骨骼肌对葡萄糖的摄取。
J Biol Methods. 2022 Aug 23;9(3):e162. doi: 10.14440/jbm.2022.385. eCollection 2022.
2
Exercise restores skeletal muscle glucose delivery but not insulin-mediated glucose transport and phosphorylation in obese subjects.运动可恢复肥胖受试者骨骼肌的葡萄糖供应，但不能恢复胰岛素介导的葡萄糖转运及磷酸化过程。
J Clin Endocrinol Metab. 2006 Sep;91(9):3394-403. doi: 10.1210/jc.2006-0269. Epub 2006 Jun 13.
3
Effect of a tumour-derived lipid-mobilising factor on glucose and lipid metabolism in vivo.一种肿瘤源性脂质动员因子对体内葡萄糖和脂质代谢的影响。
Br J Cancer. 2002 Aug 27;87(5):580-4. doi: 10.1038/sj.bjc.6600493.
4
Glucose uptake and lumped constant variability in normal human hearts determined with [18F]fluorodeoxyglucose.用[18F]氟脱氧葡萄糖测定正常人心脏的葡萄糖摄取和集总常数变异性。
J Nucl Cardiol. 1997 Mar-Apr;4(2 Pt 1):125-32. doi: 10.1016/s1071-3581(97)90061-1.
5
Fuel utilization in colonocytes of the rat.大鼠结肠细胞中的燃料利用情况。
Biochem J. 1985 Nov 1;231(3):713-9. doi: 10.1042/bj2310713.
6
A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat.一种在麻醉大鼠的骨骼肌和白色脂肪组织中定量体内葡萄糖利用的方法。
Biochem J. 1985 May 15;228(1):103-10. doi: 10.1042/bj2280103.
7
Compartmentation of hexokinase in rat heart. A critical factor for tracer kinetic analysis of myocardial glucose metabolism.大鼠心脏中己糖激酶的区室化。心肌葡萄糖代谢示踪动力学分析的关键因素。
J Clin Invest. 1992 Nov;90(5):1972-7. doi: 10.1172/JCI116076.
本文引用的文献
1
Regulation of glucose uptake by muscle. 6. Fructose 1,6-diphosphatase activity of rat heart and rat diaphragm.肌肉对葡萄糖摄取的调节。6. 大鼠心脏和大鼠膈肌的果糖1,6 -二磷酸酶活性。
Biochem J. 1962 May;83(2):387-92. doi: 10.1042/bj0830387.
2
Acid phosphatase. VI. Kinetic properties of purified yeast and erythrocyte phosphomonoesterase.酸性磷酸酶。VI. 纯化的酵母和红细胞磷酸单酯酶的动力学性质。
Arch Biochem Biophys. 1956 Mar;61(1):197-210. doi: 10.1016/0003-9861(56)90332-0.
3
A rapid method for isolating glucose metabolites involved in substrate cycling.一种用于分离参与底物循环的葡萄糖代谢物的快速方法。
Anal Biochem. 1980 Dec;109(2):443-8. doi: 10.1016/0003-2697(80)90675-2.
4
The rate of substrate cycling between glucose and glucose 6-phosphate in muscle and fat-body of the hawk moth (Acherontia atropos) at rest and during flight.
Biochem J. 1983 Jan 15;210(1):49-54. doi: 10.1042/bj2100049.
5
Maximum activities and properties of glucose 6-phosphatase in muscles from vertebrates and invertebrates.脊椎动物和无脊椎动物肌肉中葡萄糖-6-磷酸酶的最大活性和特性。
Biochem J. 1981 Sep 15;198(3):621-9. doi: 10.1042/bj1980621.
6
A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate.一种以[U-14C]6-磷酸葡萄糖为底物测定大鼠肝脏中葡萄糖-6-磷酸酶活性的方法。
Anal Biochem. 1978 Jul 15;88(1):29-36. doi: 10.1016/0003-2697(78)90395-0.
7
Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates.脊椎动物和无脊椎动物肌肉中柠檬酸合酶以及与NAD⁺和NADP⁺相关的异柠檬酸脱氢酶的活性。
Biochem J. 1976 Mar 15;154(3):689-700. doi: 10.1042/bj1540689.
Zotero 插件