van Koppen C J, Siero H L, Rodrigues de Miranda J F, Beld A J, Ariëns E J
Biochem Biophys Res Commun. 1984 Apr 30;120(2):665-9. doi: 10.1016/0006-291x(84)91307-x.
Muscarinic receptor and beta-adrenergic receptor binding were measured simultaneously in a membrane fraction of bovine tracheal smooth muscle using [3H]-L-quinuclidinyl benzilate and [125I]-(-) iodocyanopindolol. The binding characteristics, affinity and receptor density, obtained in the double receptor assay and in the control experiments were the same within experimental error. Moreover, there appears to be neither a significant influence of an excess of d,1-propranolol on [3H]-L-quinuclidinyl benzilate binding nor a significant influence of an excess of 1-quinuclidinyl benzilate on [125I]-(-)iodocyanopindolol binding. The method is advantageous where both receptors have to be assayed and where limited amounts of biological material, like in biopsy specimen, are available.
使用[3H]-L-喹核醇基苯甲酸酯和[125I]-（-）碘氰吲哚洛尔，在牛气管平滑肌的膜部分同时测量毒蕈碱受体和β-肾上腺素能受体的结合。在双受体测定和对照实验中获得的结合特性、亲和力和受体密度在实验误差范围内是相同的。此外，过量的d，1-普萘洛尔对[3H]-L-喹核醇基苯甲酸酯结合似乎没有显著影响，过量的1-喹核醇基苯甲酸酯对[125I]-（-）碘氰吲哚洛尔结合也没有显著影响。该方法在必须同时检测两种受体且生物材料量有限（如活检标本）的情况下具有优势。
