Orosz C G, Roopenian D C
Lymphokine Res. 1984;3(1):23-30.
Phorbol myristate acetate (PMA), a reagent frequently used in vitro to bypass macrophage function and to elicit cytokines, has a variety of effects on the behavior of T lymphocytes in tissue culture. For example, PMA in concentrations greater than 10(-10)M is highly comitogenic with phytohemagglutinin (PHA) for C3H/HeJ thymocytes, and significantly potentiates lymphokine-induced proliferative responses of long-term MLC cells, cloned cytolytic T cells, and interleukin-2 (IL-2)-dependent T-cell lines. Since these activated T-lymphocyte populations are used to detect IL-2, PMA can interfere with accurate assessment of IL-2 concentration by quantitative bioassay. Further, if greater than 10(-10) M PMA remains as a contaminant in PMA-induced lymphokine preparations, it can mediate lymphokine-like biologic activity, and, therefore, obscure interpretation of experiments involving lymphocyte-lymphokine interactions.
佛波醇肉豆蔻酸酯乙酸酯(PMA)是一种常用于体外绕过巨噬细胞功能并引发细胞因子的试剂,它对组织培养中T淋巴细胞的行为有多种影响。例如,浓度大于10^(-10)M的PMA与植物血凝素(PHA)对C3H/HeJ胸腺细胞具有高度协同致有丝分裂作用,并显著增强长期混合淋巴细胞培养(MLC)细胞、克隆的细胞毒性T细胞和白细胞介素-2(IL-2)依赖性T细胞系的淋巴因子诱导的增殖反应。由于这些活化的T淋巴细胞群体用于检测IL-2,PMA会干扰通过定量生物测定法对IL-2浓度的准确评估。此外,如果在PMA诱导的淋巴因子制剂中残留大于10^(-10)M的PMA作为污染物,它可以介导类似淋巴因子的生物活性,因此会模糊涉及淋巴细胞-淋巴因子相互作用的实验的解释。