鉴定24,25,26,27-四降-23-羟基维生素D3为24,25-二羟基维生素D3的肾脏代谢产物。

Identification of 24,25,26,27-tetranor-23-hydroxyvitamin D3 as a product of the renal metabolism of 24,25-dihydroxyvitamin D3.

作者信息

Jones G, Kano K, Yamada S, Furusawa T, Takayama H, Suda T

出版信息

Biochemistry. 1984 Jul 31;23(16):3749-54. doi: 10.1021/bi00311a028.

Abstract

By cochromatography, mass spectrometry, and chemical derivatization, we have shown that a metabolite isolated from the perfused rat kidney incubated with 24-(R),25-dihydroxyvitamin D3 is indistinguishable from chemically synthesized 24,25,26,27-tetranor-23-hydroxyvitamin D3. The new metabolite is also produced from 24-oxo-25-hydroxyvitamin D3 but not from 23(S),25-dihydroxyvitamin D3. Enzymes required for the synthesis of the new metabolite are absent in the vitamin D deplete animal but are induced along with the 25-hydroxyvitamin-D3 24-hydroxylase by vitamin D repletion. The pathway of 24,25-dihydroxyvitamin D3 metabolism in the perfused kidney is stimulated by pre-treatment of the rat with large doses of vitamin D3, suggesting that the pathway is a degradative one.

摘要

通过共色谱法、质谱分析法和化学衍生化方法，我们发现从用24-(R),25-二羟基维生素D3孵育的灌注大鼠肾脏中分离出的一种代谢物与化学合成的24,25,26,27-四降-23-羟基维生素D3无法区分。这种新的代谢物也由24-氧代-25-羟基维生素D3产生，但不由23(S),25-二羟基维生素D3产生。维生素D缺乏的动物体内不存在合成这种新代谢物所需的酶，但随着维生素D补充，这些酶会与25-羟基维生素D3 24-羟化酶一起被诱导产生。用大剂量维生素D3预处理大鼠会刺激灌注肾脏中24,25-二羟基维生素D3的代谢途径，这表明该途径是一种降解途径。