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[铬与白细胞的结合]

[Chromium binding to leukocytes].

作者信息

Sertoli A, Spadoni S, Giorgini S, Lombardi P

出版信息

Boll Ist Sieroter Milan. 1984 Jul 31;63(3):207-12.

PMID:6334531
Abstract

The experiment has been done on 63 patients affected by allergic contact dermatitis and sensitized to potassium dichromate and on 76 non allergic subjects to control: if leukocytes (separated in emagel), mononuclear cells (separated in lymphoprep) and T-lymphocytes (separated through rosettes with neuraminidase) bind the 51Cr, if this binding depends on the valence (Cr3+ and Cr6+) and on the type of cells and if it is conditioned by the hypersensitivity. Through incubation of the cells with the tracing at 4 degrees C and in different times, it has been demonstrated that the binding takes place, it depends on the valence and, in certain conditions it is bigger for the mononuclear cells and the T-lymphocytes in allergic subjects.

摘要

该实验对63例患有过敏性接触性皮炎且对重铬酸钾致敏的患者以及76例非过敏受试者进行了对照研究:观察白细胞(在伊格尔培养基中分离)、单核细胞(在淋巴细胞分离液中分离)和T淋巴细胞(通过神经氨酸酶玫瑰花结法分离)是否结合51Cr,这种结合是否取决于价态(Cr3+和Cr6+)和细胞类型,以及是否受超敏反应影响。通过在4℃下将细胞与示踪剂孵育不同时间,结果表明结合确实发生,其取决于价态,并且在某些情况下,过敏受试者的单核细胞和T淋巴细胞的结合更强。

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