悬滴微量分析法中绵羊外周血T淋巴细胞对PHA和LPS的促有丝分裂反应。
Mitogenic responses of sheep peripheral blood T-lymphocytes to PHA and LPS in a hanging drop microassay.
作者信息
Mlangwa J E
出版信息
Acta Vet Scand. 1984;25(4):548-60. doi: 10.1186/BF03546922.
Culture conditions for studying sheep PBL responses to mitogens (PHA and LPS) in a hanging drop micro-assay were investigated. Using those conditions, the responses of putative sheep T-cells (vide infra) to the two mitogens were examined. The results show that sheep PBL at a cell density of 1–2×10/ml responded optimally to LPS (50 (µg/ml) and PHA (3.125 µg/ml) when cultured in RPMI-1640 in the presence of 2ME, 5% FGS for 48 to 146 h. The cells were cultured as hanging drops (20 µl) in Terasaki plates. Lamb serum failed to support responses to LPS. T-cells prepared by the nylon wool technique and Helix pomatia-Sepharose-6MB column chromatography responsed vigorously to PHA, but responses to LPS were weak.
研究了在悬滴微量试验中用于研究绵羊外周血淋巴细胞（PBL）对丝裂原（PHA和LPS）反应的培养条件。利用这些条件，检测了假定的绵羊T细胞（见下文）对这两种丝裂原的反应。结果表明，当在含有2ME、5%胎牛血清（FGS）的RPMI-1640培养基中于悬滴（20μl）中在Terasaki板上培养48至146小时时，细胞密度为1 - 2×10⁶/ml的绵羊PBL对LPS（50μg/ml）和PHA（3.125μg/ml）反应最佳。羔羊血清不能支持对LPS的反应。通过尼龙棉技术和光滑双脐螺-琼脂糖-6MB柱色谱法制备的T细胞对PHA反应强烈，但对LPS反应较弱。
Zotero 插件