用于完全回收结合抗体的酸性基质洗脱方法的改进。
Modification of acid stromal elution method for complete recovery of bound antibodies.
作者信息
Araszkiewicz P, Huff S R, Szymanski I O
出版信息
Transfusion. 1983 Jan-Feb;23(1):72-4. doi: 10.1046/j.1537-2995.1983.23183147313.x.
Abstract
Erythrocyte-bound antibodies could be eluted from stroma almost completely using a modification of the method of Jenkins and Moore. (1) In the modified method, eight volumes of 0.1M glycine, pH 3.0, were added to one volume of stroma and incubated at 45 degrees C for 30 minutes. This method was optimal for autoantibodies and all alloantibodies studied.
摘要
采用詹金斯和摩尔方法的改良版，可几乎完全从红细胞基质中洗脱结合红细胞的抗体。（1）在改良方法中，向一份体积的基质中加入八份体积的0.1M甘氨酸（pH 3.0），并在45℃下孵育30分钟。该方法对研究的自身抗体和所有同种抗体而言是最佳的。
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