赖氨酰缓激肽刺激培养的人成纤维细胞中的钠离子内流和DNA合成。

Lys-bradykinin stimulates Na+ influx and DNA synthesis in cultured human fibroblasts.

作者信息

Owen N E, Villereal M L

出版信息

Cell. 1983 Mar;32(3):979-85. doi: 10.1016/0092-8674(83)90082-x.

Abstract

The effect of Lys-bradykinin on net Na+ influx in serum-deprived cultured human fibroblasts (HSWP cells) was measured. It was found that Lys-bradykinin stimulates net Na+ influx with a K1/2 of 1 nM. When Lys-bradykinin was combined with epidermal growth factor, vasopressin and insulin, the net Na+ influx stimulated was comparable with that measured in response to 10% serum. The above combination of growth factors also was found to stimulate DNA synthesis to 92% of the serum-stimulated levels in HSWP cells and to support cell growth over a period of 6 days. In addition, it was observed that the Na+ influx stimulated by Lys-bradykinin or by the combination of four growth factors was completely inhibited by the amiloride analog benzamil. Thus Lys-bradykinin presumably stimulates the same Na+ transport system as is stimulated by serum. Finally, the present results suggest that an increase in Na+ influx always accompanies DNA synthesis in HSWP cells. On the basis of these observations, it can be hypothesized that Na+ influx is a necessary event to stimulate DNA synthesis.

摘要

测定了赖氨酰缓激肽对血清饥饿培养的人成纤维细胞（HSWP细胞）净Na⁺内流的影响。发现赖氨酰缓激肽以1 nM的半数最大活性浓度（K1/2）刺激净Na⁺内流。当赖氨酰缓激肽与表皮生长因子、血管加压素和胰岛素联合使用时，刺激的净Na⁺内流与对10%血清反应时测得的相当。还发现上述生长因子组合能将HSWP细胞中的DNA合成刺激至血清刺激水平的92%，并在6天内支持细胞生长。此外，观察到赖氨酰缓激肽或四种生长因子组合刺激的Na⁺内流被氨氯地平类似物苯甲米完全抑制。因此，赖氨酰缓激肽可能刺激与血清刺激相同的Na⁺转运系统。最后，目前的结果表明，HSWP细胞中DNA合成总是伴随着Na⁺内流增加。基于这些观察结果，可以假设Na⁺内流是刺激DNA合成的必要事件。