一种由墨菲-斯特姆淋巴肉瘤酸性蛋白酶作用于大鼠血浆激肽原形成的新血管肽。

A new vasopeptide formed by the action of a Murphy-Sturm lymphosarcoma acid protease on rat plasma kininogen.

作者信息

Bedi G S, Balwierczak J, Back N

出版信息

Biochem Biophys Res Commun. 1983 Apr 29;112(2):621-8. doi: 10.1016/0006-291x(83)91509-7.

Abstract

A new vasoactive peptide, formed by the action of a Murphy-Sturm lymphosarcoma acid protease on rat plasma kininogen was purified by gel filtration on Sephadex G-50 (fine) and fractions assayed on the isolated rat uterus for smooth muscle stimulating activity. The most active fraction was purified further by CM-cellulose chromatography. High voltage electrophoresis showed the peptide to be one component (Mgly 2.49) with an electrophoretic mobility different from bradykinin, lysyl-bradykinin and methionyl-lysyl-bradykinin. The molecular weight of the peptide was estimated on Sephadex G-25 column to be 1460. The amino acid composition was determined and the carboxyl terminal sequence identified by carboxypeptidase Y treatment to be Pro-Phe-Arg-Leu. Dansyl-Edman procedure yielded an amino terminal sequence of Ile-Ser-Arg-Pro. The peptide produced a dose-dependent contraction of the isolated guinea pig anterior mesenteric vein and relaxed the rabbit superior mesenteric artery contracted by phenylephrine.

摘要

一种新的血管活性肽是由墨菲 - 斯特姆淋巴肉瘤酸性蛋白酶作用于大鼠血浆激肽原形成的，通过在葡聚糖G - 50（细）上进行凝胶过滤进行纯化，并在离体大鼠子宫上检测各组分的平滑肌刺激活性。活性最高的组分通过CM - 纤维素色谱进一步纯化。高压电泳显示该肽为一个组分（迁移率2.49），其电泳迁移率与缓激肽、赖氨酰缓激肽和甲硫氨酰赖氨酰缓激肽不同。在葡聚糖G - 25柱上估计该肽的分子量为1460。测定了氨基酸组成，并通过羧肽酶Y处理鉴定羧基末端序列为Pro - Phe - Arg - Leu。丹磺酰 - 埃德曼法得到的氨基末端序列为Ile - Ser - Arg - Pro。该肽使离体豚鼠肠系膜前静脉产生剂量依赖性收缩，并使由去氧肾上腺素收缩的兔肠系膜上动脉舒张。