Mori Y, Niwa T, Toyoshi K, Hirano K, Sugiura M
Mutat Res. 1983 Aug;121(2):95-102. doi: 10.1016/0165-7992(83)90106-9.
The mutagenicities of 3'-methyl-N,N-dimethyl-4-aminoazobenzene (3'-Me-DAB) and 3'-CH2OH-DAB, potent hepatocarcinogens, activated by rat-liver S9 were compared with those of their isomers (2'- or 4'-substituted DAB) and with those obtained with liver S9 from mice, hamsters and man. All 6 aminoazo dyes showed positive mutagenicity on both strains TA98 and TA100 in the presence of liver S9 from rats pretreated with polychlorinated biphenyls (PCB) whereas 3'-Me-DAB and 3'-CH2OH-DAB were negative in the presence of S9 from other organs of rats and human liver. 3'-Me-DAB and 3'-CH2OH-DAB also showed negative or only a weak mutagenicity in the presence of liver S9 from non-treated animals. Treatment of the muta-carcinogens by liver S9 from PCB-treated mice or hamsters exerted mutagenicity on TA98, but less than that seen with rat-liver S9. The activity of 3'-Me-DAB in the presence of female rat-liver S9 was lower than that obtained with the male. Thus a specificity in the aminoazo dye carcinogenesis in regard to species, sex and organ was also observed in the mutagenic effects of 3'-Me-DAB on Salmonella.
比较了强致癌剂3'-甲基-N,N-二甲基-4-氨基偶氮苯（3'-Me-DAB）和3'-CH2OH-DAB经大鼠肝脏S9激活后的致突变性，以及它们的异构体（2'-或4'-取代的DAB）的致突变性，并与用小鼠、仓鼠和人的肝脏S9获得的致突变性进行了比较。在经多氯联苯（PCB）预处理的大鼠的肝脏S9存在下，所有6种氨基偶氮染料在TA98和TA100菌株上均显示出阳性致突变性，而在大鼠其他器官和人肝脏的S9存在下，3'-Me-DAB和3'-CH2OH-DAB呈阴性。在未处理动物的肝脏S9存在下，3'-Me-DAB和3'-CH2OH-DAB也显示出阴性或仅微弱的致突变性。用经PCB处理的小鼠或仓鼠的肝脏S9处理诱变致癌物，对TA98有诱变作用，但低于大鼠肝脏S9的诱变作用。在雌性大鼠肝脏S9存在下，3'-Me-DAB的活性低于雄性大鼠肝脏S9的活性。因此，在3'-Me-DAB对沙门氏菌的诱变作用中，也观察到了氨基偶氮染料致癌作用在物种、性别和器官方面的特异性。
