Algvere P, Kock E
Am J Ophthalmol. 1983 Sep;96(3):345-53. doi: 10.1016/s0002-9394(14)77826-2.
We injected 20-nm carbon particles into the vitreous of 14 young rabbits that were killed eight to ten weeks later. Histologic examination showed partial posterior vitreous detachments, epiretinal cellular proliferation, and membranes in all eyes and retinal detachments in five eyes. Electron microscopy disclosed that the epiretinal membranes were formed mainly by Müller cell expansions, astrocytes, and macrophages. Müller cells penetrated the internal limiting membrane and removed carbon particles from the vitreous by endocytosis. The experiments indicated that gaps are produced in the internal limiting membrane by glial cells and macrophages that invade the vitreous in an attempt to remove foreign material. The experimental epiretinal membranes resembled idiopathic preretinal gliosis or macular pucker.
我们将20纳米的碳颗粒注入14只幼兔的玻璃体中，8至10周后处死这些兔子。组织学检查显示，所有眼睛均出现部分玻璃体后脱离、视网膜前细胞增殖和膜形成，5只眼睛出现视网膜脱离。电子显微镜检查发现，视网膜前膜主要由米勒细胞扩张、星形胶质细胞和巨噬细胞形成。米勒细胞穿透内界膜，通过内吞作用从玻璃体中清除碳颗粒。实验表明，胶质细胞和巨噬细胞侵入玻璃体试图清除异物时，会在内界膜上产生间隙。实验性视网膜前膜类似于特发性视网膜前胶质增生或黄斑皱襞。
