Shipley G D, Childs C B, Volkenant M E, Moses H L
Cancer Res. 1984 Feb;44(2):710-6.
The effects of epidermal growth factor (EGF), insulin, and a transforming growth factor from fetal bovine serum on protein synthesis, DNA synthesis, and morphology in confluent cultures of AKR-2B mouse cells have been examined. The cells were plated and grown to confluence in serum-containing medium, and then the medium was changed to MCDB 402 containing no undefined supplements for 2 days before the addition of the polypeptides to be tested. Under these conditions, all of the factors listed above stimulated rapid increases in protein synthesis. When added to cultures singly, however, only EGF stimulated significant DNA synthesis. The effect of EGF on DNA synthesis was highly synergistic with insulin. Fetal serum transforming growth factor activity, which stimulated multiple rounds of cell replication in soft agar in serum-containing medium, did not stimulate DNA synthesis in serum-free monolayer cultures of these cells when added alone or in combination with EGF or insulin. Fetal serum transforming growth factor did, however, stimulate a rapid change in the morphology of these cells from flat, nonoverlapping cells to bipolar, multilayered cultures when added to the serum-free medium.
研究了表皮生长因子（EGF）、胰岛素以及胎牛血清中的一种转化生长因子对AKR-2B小鼠细胞汇合培养物中蛋白质合成、DNA合成及细胞形态的影响。将细胞接种于含血清培养基中生长至汇合，然后在添加待测试的多肽前2天，将培养基更换为不含未定义补充物的MCDB 402培养基。在这些条件下，上述所有因子均刺激蛋白质合成快速增加。然而，单独添加到培养物中时，只有EGF刺激显著的DNA合成。EGF对DNA合成的作用与胰岛素具有高度协同性。胎牛血清转化生长因子活性在含血清培养基中能刺激软琼脂中细胞进行多轮复制，但单独添加或与EGF或胰岛素联合添加时，在这些细胞的无血清单层培养物中并不刺激DNA合成。不过，当添加到无血清培养基中时，胎牛血清转化生长因子确实能刺激这些细胞的形态从扁平、不重叠细胞快速转变为双极、多层培养物。
