Ploemacher R E, Brons N H
Cell Tissue Kinet. 1984 Jan;17(1):1-12. doi: 10.1111/j.1365-2184.1984.tb00563.x.
A tentative characterization of haemopoietic stem cells with respect to their organ distribution, seeding fraction and colony formation in the spleen, radiosensitivity and humoral regulation was attempted in mice heterozygous for the mutant allele Slj and in their normal littermates. Slj/+ mice were characterized by a deficient CFU-s content of the blood and spleen and had slightly lower femoral CFU-s numbers. This CFU-s distribution could not be explained by differences in seeding efficiency 'f' between CFU-s of Slj/+ and +/+ origin in lethally irradiated recipients used in the CFU-s assay. The seeding fraction of CFU-s of +/+ origin did not differ in +/+ and Slj/+ recipients. However, in irradiated Slj/+ recipient mice a 30% decrease was observed in the number of the colonies derived from splenic and femoral CFU-s of both +/+ and Slj/+ origin. The serum level of SHSF (splenic haemopoiesis stimulating factor) was decreased in Slj/+ mice, but significantly increased in Sl/Sld mice, as compared to their respective normal +/+ littermates. Endogenous colony formation in Slj/+ spleens was deficient in comparison to that observed in +/+ spleens, and distinct sex differences were observed. However, mutant and normal CFU-s from spleen and bone marrow had a similar survival following in-vitro gamma irradiation. Femurs and spleens of both Slj/+ and +/+ origin were implanted into both Slj/+ and +/+ hosts. Six weeks later the Slj/+ grafts contained less CFU-s than the +/+ grafts. These data show that the splenic stroma of Slj/+ mice is not defective in its capacity to lodge injected CFU-s but is deficient in its ability to maintain CFU-s under 'steady-state' conditions and stimulate their colony formation in a 'perturbed state'. Some of the characteristics of Slj/+ mice segregate them from Sl/Sld mice, i.e. a deficient splenic CFU-s content, normal seeding fractions 'f' of CFU-s from spleen and bone marrow in the presence of an almost compensated anemia, and decreased serum levels of SHSF. The study of the Slj trait may be a useful extension of the current Sl/Sld model for exploration of hereditary defects in haematopoietic stroma.
在携带突变等位基因Slj的杂合子小鼠及其正常同窝仔鼠中，尝试对造血干细胞的器官分布、植入分数、在脾脏中的集落形成、放射敏感性和体液调节进行初步表征。Slj/+小鼠的特征是血液和脾脏中CFU-s含量不足，股骨CFU-s数量略低。在CFU-s测定中使用的经致死性照射的受体中，Slj/+和+/+来源的CFU-s在植入效率“f”上的差异无法解释这种CFU-s分布情况。+/+来源的CFU-s在+/+和Slj/+受体中的植入分数没有差异。然而，在经照射的Slj/+受体小鼠中，观察到来自+/+和Slj/+来源的脾脏和股骨CFU-s的集落数量减少了30%。与各自正常的+/+同窝仔鼠相比，Slj/+小鼠的SHSF（脾脏造血刺激因子）血清水平降低，但在Sl/Sld小鼠中显著升高。与在+/+脾脏中观察到的情况相比，Slj/+脾脏中的内源性集落形成不足，并且观察到明显的性别差异。然而，来自脾脏和骨髓的突变型和正常CFU-s在体外γ照射后具有相似的存活率。将Slj/+和+/+来源的股骨和脾脏植入Slj/+和+/+宿主中。六周后，Slj/+移植物中的CFU-s比+/+移植物中的少。这些数据表明，Slj/+小鼠的脾脏基质在容纳注射的CFU-s的能力上没有缺陷，但在“稳态”条件下维持CFU-s并在“扰动状态”下刺激其集落形成的能力不足。Slj/+小鼠的一些特征将它们与Sl/Sld小鼠区分开来，即脾脏CFU-s含量不足、在几乎代偿性贫血的情况下脾脏和骨髓中CFU-s的正常植入分数“f”以及SHSF血清水平降低。对Slj性状的研究可能是当前Sl/Sld模型用于探索造血基质遗传缺陷的有益扩展。
