大鼠纹状体细胞培养物中亮氨酸脑啡肽定位与[3H]γ-氨基丁酸摄取的同步测定。

Simultaneous determination of leu-enkephalin localization and [3H] gamma-aminobutyric acid uptake in rat striatal cell cultures.

作者信息

Mazurkiewicz J E, Messer A

出版信息

Cell Mol Neurobiol. 1983 Sep;3(3):255-62. doi: 10.1007/BF00710951.

DOI:10.1007/BF00710951

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11572923/

Abstract

The purpose of this study was to investigate the coexistence of gamma-aminobutyric acid (GABA) and leu-enkephalin in single neurons from the corpus striatum. Monolayer cell cultures, started from newborn rat corpus striatum, were grown in serum-free medium and examined using GABA autoradiography and leu-enkephalin immunocytochemistry in a double-label protocol. Examples of cells were found which were positive for one or the other neurotransmitter or for neither transmitter, but not for both. Furthermore, cells which appear similar by morphological criteria alone differed in transmitter specificity. We conclude that the two transmitters tested are not localized within single cells and that morphology alone is inadequate to identify functional cell classes in this area.

摘要

本研究的目的是调查纹状体单个神经元中γ-氨基丁酸（GABA）和亮氨酸脑啡肽的共存情况。从新生大鼠纹状体开始的单层细胞培养物在无血清培养基中生长，并采用双标记方案，通过GABA放射自显影和亮氨酸脑啡肽免疫细胞化学进行检测。发现了一些细胞实例，它们对一种或另一种神经递质呈阳性，或者对两种递质均呈阴性，但没有对两种递质都呈阳性的。此外，仅根据形态学标准看似相似的细胞在递质特异性方面存在差异。我们得出结论，所检测的两种递质并非定位于单个细胞内，仅靠形态学不足以识别该区域的功能性细胞类别。

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本文引用的文献

1

The distribution of enkephalin-immunoreactive cell bodies in the rat central nervous system.大鼠中枢神经系统中脑啡肽免疫反应性细胞体的分布。

Neurosci Lett. 1977 Jun;5(1-2):25-31. doi: 10.1016/0304-3940(77)90160-4.

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Immunohistochemical studies using antibodies to leucine-enkephalin: initial observations on the nervous system of the rat.使用抗亮氨酸脑啡肽抗体的免疫组织化学研究：对大鼠神经系统的初步观察。

Neuroscience. 1976 Aug;1(4):349-51. doi: 10.1016/0306-4522(76)90063-4.

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Growth of dissociated rat cerebellar cells using serum-free supplemented media and varied transferrin concentrations.使用无血清补充培养基和不同转铁蛋白浓度培养解离的大鼠小脑细胞。

Cell Mol Neurobiol. 1981 Mar;1(1):99-114. doi: 10.1007/BF00736042.

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Primary monolayer cultures of the rat corpus striatum: morphology and properties related to acetylcholine and gamma-aminobutyrate.大鼠纹状体的原代单层培养：与乙酰胆碱和γ-氨基丁酸相关的形态学和特性

Neuroscience. 1981;6(12):2677-87. doi: 10.1016/0306-4522(81)90112-3.

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The distribution and functions of the enkephalins.脑啡肽的分布与功能。

J Histochem Cytochem. 1980 Aug;28(8):903-17. doi: 10.1177/28.8.7003009.

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Immunocytochemical localization of enkephalin in the neostriatum of rat brain: a light and electron microscopic study.大鼠脑新纹状体中脑啡肽的免疫细胞化学定位：光镜和电镜研究

J Comp Neurol. 1980 Feb 15;189(4):721-40. doi: 10.1002/cne.901890408.

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Leucine-enkephalin immunoreactivity: localization in rat brain cells in culture.

Brain Res. 1980 Jan 13;181(2):465-9. doi: 10.1016/0006-8993(80)90631-9.

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Synaptic connections of enkephalin-immunoreactive nerve terminals in the neostriatum: a correlated light and electron microscopic study.新纹状体中脑啡肽免疫反应性神经终末的突触连接：光镜和电镜相关研究

J Neurocytol. 1982 Oct;11(5):779-807. doi: 10.1007/BF01153519.

9

Demonstration of enkephalin-, substance P- and glutamate decarboxylase-like immunoreactivity in cultured cells derived from newborn rat neostriatum.新生大鼠新纹状体来源的培养细胞中脑啡肽、P物质和谷氨酸脱羧酶样免疫反应性的显示

Histochemistry. 1980;69(2):169-79. doi: 10.1007/BF00533134.

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Peptidergic neurones.肽能神经元。

Nature. 1980 Apr 10;284(5756):515-21. doi: 10.1038/284515a0.

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