Bockus B J, Stiles C D
Exp Cell Res. 1984 Jul;153(1):186-97. doi: 10.1016/0014-4827(84)90460-9.
Addition of ng/ml quantities of the platelet-derived growth factor (PDGF) component of serum to the culture medium of quiescent monolayers of BALB/c-3T3 cells rapidly altered the actomyosin cytoskeletal system. PDGF triggered dispersal of microfilament bundles and induced phosphorylation of the 20 kD myosin light chain within 5 min of addition. Additional cytoskeletal responses followed the addition of insulin and of epidermal growth factor (EGF). Insulin alone induces a transient breakdown of well defined microtubule filaments. EGF and insulin together induced moderate perturbation of the cytoplasmic network of intermediate filaments which then reformed within a 90-180 min time frame. This response, however, was greatly enhanced in cells which were pretreated with PDGF and it appears to be a secondary event occurring as a consequence of cell growth. The PDGF-induced disruption of microfilament bundles could be blocked by phalloidin with no apparent inhibition of the mitogenic response. Insulin alone is only weakly mitogenic for 3T3 cells. The data thus indicate that the architecture of two discrete cytoskeletal elements (microtubules and microfilaments) is regulated directly by separate serum growth factors (insulin and PDGF). However the disruption of microfilament architecture by PDGF does not seem necessary for induction of cell growth and the disruption of microtubule filaments by insulin is not sufficient.
向静止的BALB/c-3T3细胞单层培养基中添加纳克/毫升量的血清血小板衍生生长因子（PDGF），会迅速改变肌动球蛋白细胞骨架系统。添加PDGF后5分钟内，微丝束分散，并诱导20 kD肌球蛋白轻链磷酸化。添加胰岛素和表皮生长因子（EGF）后会出现其他细胞骨架反应。单独的胰岛素会诱导明确的微管丝短暂解体。EGF和胰岛素共同作用会引起中间丝细胞质网络的中度扰动，随后在90 - 180分钟的时间范围内重新形成。然而，在用PDGF预处理的细胞中，这种反应会大大增强，并且这似乎是细胞生长导致的继发事件。鬼笔环肽可阻断PDGF诱导的微丝束破坏，而对促有丝分裂反应无明显抑制作用。单独的胰岛素对3T3细胞的促有丝分裂作用较弱。因此，数据表明两种离散的细胞骨架成分（微管和微丝）的结构直接受不同的血清生长因子（胰岛素和PDGF）调节。然而，PDGF对微丝结构的破坏似乎不是诱导细胞生长所必需的，而胰岛素对微管丝的破坏也不足以诱导细胞生长。
