Friedman H, Warren G
Proc Soc Exp Biol Med. 1984 Sep;176(4):366-70. doi: 10.3181/00379727-176-41884.
Treatment of mice with muramyl dipeptide, a known immunoadjuvant, resulted in marked augmentation of the phagocytic activity of peritoneal macrophages incubated in vitro with Escherichia coli. Even greater phagocytosis occurred when the E. coli were pretreated for 2 hr with subinhibitory concentrations of the semisynthetic penicillins cyclacillin or ampicillin, but not penicillin G to which they were resistant. The antibiotic-pretreated E. coli were more rapidly ingested by the macrophages derived from MDP-treated mice as compared to similar cells from normal mice. Optimum augmentation of phagocytosis of untreated or antibiotic-pretreated E. coli occurred 2 to 3 days after administration of MDP to the mice. Similar augmentation of phagocytosis occurred by treating cultures of peritoneal macrophages from normal mice in vitro with MDP prior to incubation with the antibiotic-pretreated bacteria. These results indicate that macrophages from MDP stimulated mice interact with antibiotic-pretreated bacteria to a greater extent than with untreated E. coli, resulting in increased phagocytosis and killing of the bacteria.
用已知的免疫佐剂胞壁酰二肽处理小鼠，导致在体外与大肠杆菌一起孵育的腹膜巨噬细胞的吞噬活性显著增强。当大肠杆菌用亚抑制浓度的半合成青霉素环青霉素或氨苄青霉素预处理2小时时，会发生更大程度的吞噬作用，但对它们耐药的青霉素G则不会。与来自正常小鼠的类似细胞相比，经抗生素预处理的大肠杆菌被来自MDP处理小鼠的巨噬细胞摄取得更快。在给小鼠施用MDP后2至3天，未处理或经抗生素预处理的大肠杆菌的吞噬作用达到最佳增强。在用抗生素预处理的细菌孵育之前，用MDP体外处理正常小鼠的腹膜巨噬细胞培养物，也会发生类似的吞噬作用增强。这些结果表明，来自MDP刺激小鼠的巨噬细胞与经抗生素预处理的细菌的相互作用比与未处理的大肠杆菌的相互作用更大，从而导致细菌的吞噬作用和杀伤增加。
