糖鞘脂印迹法：一种薄层色谱分离后的免疫检测方法。
Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatography.
作者信息
Towbin H, Schoenenberger C, Ball R, Braun D G, Rosenfelder G
出版信息
J Immunol Methods. 1984 Sep 4;72(2):471-9. doi: 10.1016/0022-1759(84)90015-2.
A method for detecting glycosphingolipids (GSL) in situ after thin layer chromatography is described. The separated GSL are transferred by diffusion to nitrocellulose. The replica is incubated with poly- or monoclonal antibodies and bound antibodies are detected with second antibodies coupled to peroxidase. Advantages of the procedure are its speed, the non-radioactive detection method, and its suitability for screening applications. In addition, small scale affinity purification of antibodies from the replicas is possible. The presence of Forssman antigen in mouse tissues and the reaction of monoclonal antibodies with human GSL is demonstrated.
本文描述了一种在薄层色谱后原位检测糖鞘脂（GSL）的方法。分离出的GSL通过扩散转移至硝酸纤维素膜上。将该复制品与多克隆或单克隆抗体一起孵育，并用与过氧化物酶偶联的二抗检测结合的抗体。该方法的优点包括速度快、采用非放射性检测方法以及适用于筛选应用。此外，还可以从复制品中进行小规模的抗体亲和纯化。文中展示了小鼠组织中福斯曼抗原的存在以及单克隆抗体与人类GSL的反应。
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