Heinonen J, Pulkkinen L, Karjalainen M, Kukko E
Biochem Int. 1983 Mar;6(3):403-8.
Hydroxyurea not only inhibited the reduction of ribonucleotides, but also gave rise to need of exogenous methionine and thymidine for full growth in the cultures of Escherichia coli K12. In vitro the drug inhibited the formation of 5,10-methylenetetrahydrofolate by serine hydroxymethyltransferase in the cell extracts, which suggests that this reaction is a secondary target of hydroxyurea in the cells of E. coli. The syntheses of thymine nucleotides and methionine were also the most sensitive targets of hydroxylamine in the cultures of E. coli.
羟基脲不仅抑制核糖核苷酸的还原，还导致大肠杆菌K12培养物完全生长需要外源性甲硫氨酸和胸腺嘧啶核苷。在体外，该药物抑制细胞提取物中丝氨酸羟甲基转移酶形成5,10-亚甲基四氢叶酸，这表明该反应是羟基脲在大肠杆菌细胞中的次要作用靶点。胸腺嘧啶核苷酸和甲硫氨酸的合成也是大肠杆菌培养物中羟胺最敏感的作用靶点。
