Klein-Szanto A J, Pal B C, Terzaghi M, Marchok A C
Environ Health Perspect. 1984 Jun;56:75-86. doi: 10.1289/ehp.845675.
Heterotopic tracheal transplants, placed subcutaneously in syngeneic rats have been extensively used in our laboratory. The objective of these experiments was to study the toxic and/or carcinogenic effect of several compounds on the respiratory tract mucosa. This was attained by exposing the transplants to an intraluminal pettet containing the toxicant or carcinogen mixed with an adequate matrix (gelatin, beeswax, stearyl alcohol, silastic, etc.). By varying the concentration of the test chemicals, it is possible to study dose-response relationships, and by changing the pellet matrix, the effects of release rate (dose rate) can be analyzed. Several end points can be studied, such as histological changes in the mucociliary epithelium, changes in mucus secretion, tumor induction and changes in the in vitro behavior of the epithelial cells after in vivo exposure. In addition, by de-epithelializing the tracheal transplants and reseeding them with another cell population, e.g., from previously treated cell cultures or from human specimens and transplanting them subcutaneously in nude mice, completely new vistas on the effect of chemicals can be opened.
异位气管移植,将其皮下植入同基因大鼠体内,已在我们实验室中广泛使用。这些实验的目的是研究几种化合物对呼吸道黏膜的毒性和/或致癌作用。这是通过将移植物暴露于含有与适当基质(明胶、蜂蜡、硬脂醇、硅橡胶等)混合的毒物或致癌物的腔内小丸来实现的。通过改变测试化学品的浓度,可以研究剂量反应关系,并且通过改变小丸基质,可以分析释放速率(剂量率)的影响。可以研究几个终点,例如黏液纤毛上皮的组织学变化、黏液分泌的变化、肿瘤诱导以及体内暴露后上皮细胞体外行为的变化。此外,通过去除气管移植物的上皮并用另一种细胞群体重新接种,例如来自先前处理过的细胞培养物或来自人类标本的细胞群体,并将它们皮下移植到裸鼠中,可以开启关于化学品作用的全新视野。