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[Obtaining hepatic stages of Plasmodium falciparum in vitro].

作者信息

Mazier D, Beaudoin R L, Mellouk S, Druilhe P, Texier B, Trosper J, Miltgen F, Landau I, Paul C, Brandicourt O

出版信息

Ann Parasitol Hum Comp. 1984;59(5):525-6. doi: 10.1051/parasite/1984595525.

Abstract

Sporozoites of Plasmodium falciparum, obtained by membrane feeding of Anopheles freeborni or A. stephensi with cultured gametocytes, were used to infect monolayers of human hepatocytes. Fluorescent labelling with an African serum as well as Giemsa staining performed from day one to day 7 of cultures, demonstrated the presence of numerous hepatic schizonts measuring up to 40 micron.

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