大鼠胰岛中前列腺素D2、E2和F2α的存在。

Presence of prostaglandin D2, E2 and F2 alpha in rat pancreatic islets.

作者信息

Horie H, Narumiya S, Matsuyama T, Nonaka K, Tarui S

出版信息

Prostaglandins Leukot Med. 1984 Oct;16(1):39-44. doi: 10.1016/0262-1746(84)90084-2.

Abstract

The presence of prostaglandin (PG) D2, PGE2 and PGF2 alpha was demonstrated in rat pancreas and pancreatic islets by specific radioimmunoassay and their contents were measured separately. The amounts of PGD2, PGE2 and PGF2 alpha in rat pancreas were 16.1 +/- 1.1, 9.48 +/- 2.05 and 4.43 +/- 0.62(2) ng/g tissue, respectively. The amounts of PGD2, PGE2 and PGF2 alpha in rat pancreatic islets were 427 +/- 66, 482 +/- 281 and 479 +/- 41 pg/mg protein, respectively. The syntheses of several PGs from PGH2, a common intermediate for PG production, in islet homogenates were also studied. PGH2 was rapidly transformed into PGD2, PGE2 and PGF2 alpha in almost equal proportion in islet homogenates, and the transformation were proportional to the amounts of the homogenates added in the reaction.

摘要

通过特异性放射免疫分析法证实了大鼠胰腺和胰岛中存在前列腺素（PG）D2、PGE2和PGF2α，并分别测定了它们的含量。大鼠胰腺中PGD2、PGE2和PGF2α的含量分别为16.1±1.1、9.48±2.05和4.43±0.62(2) ng/g组织。大鼠胰岛中PGD2、PGE2和PGF2α的含量分别为427±66、482±281和479±41 pg/mg蛋白质。还研究了胰岛匀浆中几种由PGH2（PG产生的共同中间体）合成的PG。在胰岛匀浆中，PGH2迅速以几乎相等的比例转化为PGD2、PGE2和PGF2α，且这种转化与反应中加入的匀浆量成正比。