一种用于3-羟基-3-甲基戊二酰辅酶A还原酶的改良放射测定法。
A modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme A reductase.
作者信息
Georgopapadakou N H, Dix B A
出版信息
Lipids. 1984 Dec;19(12):966-70. doi: 10.1007/BF02534735.
PMID:6396479
Abstract
A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.
摘要
本文描述了一种用于测量3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶活性的放射性测定法。该测定法基于通过高压电泳将甲羟戊酸产物与HMG-CoA分离。此方法比基于NADPH的分光光度法更灵敏、更特异，且比现有的放射性测定法更简便。它已被用于测量酿酒酵母粗提物和人皮肤成纤维细胞中的HMG-CoA还原酶活性。
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