凝血因子VIII源性非甲/非乙型肝炎与乙型肝炎病毒的不相关性

Unrelatedness of factor VIII-derived non-A/non-B hepatitis and hepatitis B virus.

作者信息

Fields H A, Berninger M, Nath N, Davis C L, Hammer M, Margolis H S, McCaustland K A, Wheeler C M, Maynard J E, Bradley D W

出版信息

J Med Virol. 1983;11(1):59-65. doi: 10.1002/jmv.1890110108.

DOI:10.1002/jmv.1890110108

PMID:6403666

Abstract

A DNA hybridization assay was used to detect hepatitis B virus (HBV)-specific DNA sequences in extracted sera obtained from chimpanzees infected with HBV, hepatitis A virus (HAV), and a factor VIII-derived non-A/non-B (NANB) agent. The results did not reveal any HBV-DNA homology with sera obtained from animals infected with HAV or factor VIII-derived NANB. Sera obtained from two HBV-infected chimpanzees demonstrated that HBV-specific DNA could be detected during the acute phase of the disease. In addition, an HBV-specific DNA-dependent DNA polymerase assay did not demonstrate any statistically significant activity in 12 of 12 NANB acute-phase specimens or in 6 of 6 NANB chronic-phase specimens. These results suggest that the factor VIII-derived NANB agent is unrelated to HBV.

摘要

采用DNA杂交试验检测从感染乙型肝炎病毒（HBV）、甲型肝炎病毒（HAV）和一种因子VIII衍生的非甲非乙型（NANB）病原体的黑猩猩提取的血清中的HBV特异性DNA序列。结果显示，感染HAV或因子VIII衍生的NANB的动物血清与HBV-DNA无同源性。从两只感染HBV的黑猩猩获得的血清表明，在疾病急性期可检测到HBV特异性DNA。此外，一项HBV特异性依赖DNA的DNA聚合酶试验显示，12份NANB急性期标本中的12份以及6份NANB慢性期标本中的6份均未表现出任何具有统计学意义的活性。这些结果表明，因子VIII衍生的NANB病原体与HBV无关。