在无血清培养基中生产人γ干扰素。

Production of human interferon-gamma in serum-free medium.

作者信息

Tsujimoto M, Tarutani T, Ogawa K, Akashi K, Okubo Y, Higashi N

出版信息

Infect Immun. 1983 Jul;41(1):181-4. doi: 10.1128/iai.41.1.181-184.1983.

DOI:10.1128/iai.41.1.181-184.1983

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264760/

Abstract

Interferon (IFN)-gamma was produced with a high yield in cultures of human peripheral mononuclear cells by combined stimulation with OK-432 and staphylococcal enterotoxin B. Human mononuclear cells cultured in serum-free medium produced several times as much IFN as those in RPMI-1640 medium containing 10% fetal bovine serum. A synergistic effect of OK-432 and staphylococcal enterotoxin B on the production of IFN-gamma was demonstrated. Ultrogel AcA54 column chromatography of crude IFN showed a single peak with an apparent molecular weight of 43,000. Our production system for human IFN-gamma offers a feasible approach to preparation of large quantities of purified IFN-gamma for structure studies, antibody production, and clinical applications.

摘要

通过OK-432和葡萄球菌肠毒素B联合刺激，人外周血单个核细胞培养物中可高产干扰素（IFN）-γ。在无血清培养基中培养的人单核细胞产生的IFN是含10%胎牛血清的RPMI-1640培养基中培养的细胞的几倍。证实了OK-432和葡萄球菌肠毒素B对IFN-γ产生具有协同作用。粗制IFN的Ultrogel AcA54柱层析显示一个单峰，表观分子量为43,000。我们的人IFN-γ生产系统为大量制备纯化的IFN-γ用于结构研究、抗体生产和临床应用提供了一种可行的方法。

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本文引用的文献

1

The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses.

Immunol Rev. 1982;63:129-66. doi: 10.1111/j.1600-065x.1982.tb00414.x.

2

Purified human immune interferon has more potent anticellular activity than fibroblast or leukocyte interferon.

Cell Immunol. 1980 Feb;49(2):390-4. doi: 10.1016/0008-8749(80)90041-6.

3

Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells.

Nature. 1982 Oct 28;299(5886):833-6. doi: 10.1038/299833a0.

4

Induction of human gamma-interferon in lymphoid cells by Staphylococcus enterotoxin B; partial purification.

J Interferon Res. 1982;2(1):11-20. doi: 10.1089/jir.1982.2.11.

5

Induction of interferon-gamma in mouse spleen cells by OK-432, a preparation of Streptococcus pyogenes.

Cell Immunol. 1982 Mar 15;68(1):187-92. doi: 10.1016/0008-8749(82)90102-2.

6

Purification of two subspecies of human gamma (immune) interferon.

Proc Natl Acad Sci U S A. 1982 Mar;79(6):1820-4. doi: 10.1073/pnas.79.6.1820.

7

Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells.

Nucleic Acids Res. 1982 Apr 24;10(8):2487-501. doi: 10.1093/nar/10.8.2487.

8

Expression of human immune interferon cDNA in E. coli and monkey cells.

Nature. 1982 Feb 11;295(5849):503-8. doi: 10.1038/295503a0.

9

Interferon induced in human leukocytes by concanavalin A: isolation and characterization of gamma- and beta-type components.

Eur J Immunol. 1981 Nov;11(11):937-42. doi: 10.1002/eji.1830111116.

10

Stimulation of human gamma interferon production by diterpene esters.

Infect Immun. 1981 Oct;34(1):131-9. doi: 10.1128/iai.34.1.131-139.1981.

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