Elias K A, Blake C A
Biol Reprod. 1983 Jun;28(5):1107-13. doi: 10.1095/biolreprod28.5.1107.
Changes at the anterior pituitary gland level which result in follicle-stimulating hormone (FSH) release after ovariectomy in metestrous rats were investigated. Experimental rats were ovariectomized at 0900 h of metestrus and decapitated at 1000, 1100, 1300, 1500, 1700 or 1900 h of metestrus. Controls consisted of untreated rats killed at 0900 or 1700 h and rats sham ovariectomized at 0900 h and killed at 1700 h. Trunk blood was collected and the serum assayed for FSH and luteinizing hormone (LH) concentrations. The anterior pituitary gland was bisected. One-half was used to assay for FSH concentration. The other half was placed in culture medium for a 30-min preincubation and then placed in fresh medium for a 2-h incubation (basal FSH and LH release rates). The basal FSH release rate and the serum FSH concentration rose significantly by 4 h postovariectomy and remained high for an additional 6 h. The basal FSH release rate and the serum FSH concentration correlated positively (r=0.71 with 72 degrees of freedom) and did not change between 0900 and 1700 h in untreated or sham-ovariectomized rats. In contrast, the serum LH concentration and the basal LH release rate did not increase after ovariectomy. Ovariectomy had no significant effect on anterior pituitary gland FSH concentration. The results suggest that the postovariectomy rise in serum FSH concentration is the result, at least in part, of changes which cause an increase in the basal FSH secretion rate (secretion independent of the immediate presence of any hormones of nonanterior pituitary gland origin). The similarities between the selective rises in the basal FSH release rate and the serum FSH concentration in the ovariectomized metestrous rat and in the cyclic rat during late proestrus and estrus raise the possibility that an increase in the basal FSH release rate may be involved in many or all situations in which serum FSH concentration rises independently of LH.
研究了动情后期大鼠卵巢切除术后垂体前叶水平导致促卵泡激素（FSH）释放的变化。实验大鼠在动情后期09:00进行卵巢切除，并在动情后期10:00、11:00、13:00、15:00、17:00或19:00断头。对照组包括在09:00处死的未处理大鼠、在17:00处死的未处理大鼠以及在09:00进行假卵巢切除并在17:00处死的大鼠。采集躯干血，测定血清中FSH和促黄体生成素（LH）的浓度。将垂体前叶一分为二。一半用于测定FSH浓度。另一半置于培养基中预孵育30分钟，然后置于新鲜培养基中孵育2小时（基础FSH和LH释放率）。卵巢切除术后4小时，基础FSH释放率和血清FSH浓度显著升高，并在接下来的6小时内保持在较高水平。基础FSH释放率与血清FSH浓度呈正相关（自由度为72时r = 0.71），在未处理或假卵巢切除的大鼠中，09:00至17:00之间无变化。相比之下，卵巢切除术后血清LH浓度和基础LH释放率未增加。卵巢切除术对垂体前叶FSH浓度无显著影响。结果表明，卵巢切除术后血清FSH浓度升高至少部分是由于基础FSH分泌率增加（分泌不依赖于任何非垂体前叶来源激素的直接存在）所导致的变化。动情后期卵巢切除大鼠与发情前期和发情期的周期性大鼠中基础FSH释放率和血清FSH浓度的选择性升高之间的相似性，增加了基础FSH释放率升高可能参与血清FSH浓度独立于LH升高的许多或所有情况的可能性。
