Kirkpatrick C J, Mohr W, Haferkamp O
Exp Cell Biol. 1983;51(4):192-200. doi: 10.1159/000163191.
Using the Fell technique of organ culture of 8-day chick embryo femoral and tibial rudiments, the effects of indomethacin, dihomo-gamma-linolenic acid and arachidonic acid on limb rudiment linear growth and differentiation were investigated. Indomethacin (50 and 100 mumol/l) elicited a statistically significant decrease in rudiment linear growth without affecting differentiation or cell structure. Dihomo-gamma-linolenic acid and arachidonic acid, both at 100 mumol/l, exerted no effect on limb rudiment linear growth or differentiation. From previous work, it has been shown that PGA1 and PGB1 caused a marked inhibition of linear growth, PGA1 being cytotoxic. The failure of the prostaglandin (PG) precursors to reproduce these effects suggests that PGA or PGB biosynthesis in embryonic chondrocytes plays no significant role in cartilage growth regulatory mechanisms. Moreover, the growth inhibitory effect of the PG cyclooxygenase inhibitor, indomethacin, suggests that a product of arachidonic acid metabolism via the cyclooxygenase pathway may promote cartilage growth.
采用8日龄鸡胚股骨和胫骨原基的费尔器官培养技术，研究了吲哚美辛、二高-γ-亚麻酸和花生四烯酸对肢体原基线性生长和分化的影响。吲哚美辛（50和100μmol/L）可使原基线性生长在统计学上显著降低，而不影响分化或细胞结构。二高-γ-亚麻酸和花生四烯酸，均为100μmol/L，对肢体原基线性生长或分化无影响。从先前的研究工作可知，PGA1和PGB1可显著抑制线性生长，PGA1具有细胞毒性。前列腺素（PG）前体未能重现这些效应，这表明胚胎软骨细胞中PGA或PGB的生物合成在软骨生长调节机制中不起重要作用。此外，PG环氧化酶抑制剂吲哚美辛的生长抑制作用表明，花生四烯酸经环氧化酶途径代谢的产物可能促进软骨生长。
