一种用于分析四环素药物标准品、类似物、降解产物及其他杂质的高效液相色谱系统。

A high performance liquid chromatographic system for the analysis of tetracycline drug standards, analogs, degradation products and other impurities.

作者信息

Mack G D, Ashworth R B

出版信息

J Chromatogr Sci. 1978 Mar 10;16(3):93-101. doi: 10.1093/chromsci/16.3.93.

DOI:10.1093/chromsci/16.3.93

Abstract

This paper describes the high performance liquid chromatographic (HPLC) analysis of eight parent tetracycline standards: tetracycline, chlortetracycline, rolitetracycline, oxitetracycline, minocycline, doxycycline, democlocycline, methacycline; and three tetracycline epimers: epitetracycline, epianhydrotetracycline, and anhydrotetracycline. The HPLC system employs an octadecylsilane reverse phase column and an isopropanol-diethanolamine-phosphate-ammonium EDTA-water mobile phase. This system produced at least partial resolution of all eight parent compounds and many of their degradation products.

摘要

本文描述了对8种四环素母体标准品：四环素、金霉素、罗利环素、土霉素、米诺环素、多西环素、地美环素、美他环素；以及3种四环素差向异构体：表四环素、差向脱水四环素和脱水四环素的高效液相色谱（HPLC）分析。该HPLC系统采用十八烷基硅烷反相柱和异丙醇 - 二乙醇胺 - 磷酸盐 - 乙二胺四乙酸铵 - 水流动相。该系统至少能部分分离所有8种母体化合物及其许多降解产物。