• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Chemical modification of microsomal cytochrome P450: role of lysyl residues in hydroxylation activity.

作者信息

Kunz B C, Richter C

出版信息

FEBS Lett. 1983 Sep 19;161(2):311-4. doi: 10.1016/0014-5793(83)81031-x.

DOI:10.1016/0014-5793(83)81031-x
PMID:6413255
Abstract

Cytochrome P450 purified from phenobarbital-induced rat liver microsomes was acetylated at 3 lysyl residues. When reconstituted with purified NADPH-cytochrome P450 reductase, the modified cytochrome showed full activity and substrate-induced spectral changes with d-benzphetamine. With 7-ethoxycoumarin, neither enzymic activity nor binding was detected. It is concluded that the positively charged lysine residues of cytochrome P450 are important for metabolism of 7-ethoxycoumarin by cytochrome P450.

摘要

相似文献

1
Chemical modification of microsomal cytochrome P450: role of lysyl residues in hydroxylation activity.
FEBS Lett. 1983 Sep 19;161(2):311-4. doi: 10.1016/0014-5793(83)81031-x.
2
The role of cytochrome P450 lysine residues in the interaction between cytochrome P450IA1 and NADPH-cytochrome P450 reductase.
Arch Biochem Biophys. 1992 Apr;294(1):83-90. doi: 10.1016/0003-9861(92)90140-r.
3
Reduction of 7-alkoxyresorufins by NADPH-cytochrome P450 reductase and its differential effects on their O-dealkylation by rat liver microsomal cytochrome P450.NADPH-细胞色素P450还原酶对7-烷氧基试卤灵的还原作用及其对大鼠肝微粒体细胞色素P450介导的O-脱烷基反应的不同影响。
Arch Biochem Biophys. 1989 Feb 1;268(2):617-29. doi: 10.1016/0003-9861(89)90329-9.
4
Comparative study of monomeric reconstituted and membrane microsomal monooxygenase systems of the rabbit liver. II. Kinetic parameters of reductase and monooxygenase reactions.兔肝单体重组和膜微粒体单加氧酶系统的比较研究。II. 还原酶和单加氧酶反应的动力学参数。
Arch Biochem Biophys. 1992 Nov 1;298(2):403-12. doi: 10.1016/0003-9861(92)90428-y.
5
Role of lysine and arginine residues of cytochrome P450 in the interaction between cytochrome P4502B1 and NADPH-cytochrome P450 reductase.细胞色素P450中赖氨酸和精氨酸残基在细胞色素P4502B1与NADPH-细胞色素P450还原酶相互作用中的作用
Arch Biochem Biophys. 1993 Jul;304(1):257-65. doi: 10.1006/abbi.1993.1347.
6
Isolation of cytochrome P450 from hepatopancreas microsomes of the spiny lobster, Panulirus argus, and determination of catalytic activity with NADPH cytochrome P450 reductase from vertebrate liver.从多刺龙虾（Panulirus argus）肝胰腺微粒体中分离细胞色素P450，并测定其与脊椎动物肝脏中NADPH细胞色素P450还原酶的催化活性。
Arch Biochem Biophys. 1990 Oct;282(1):8-17. doi: 10.1016/0003-9861(90)90080-i.
7
Purification of rat liver microsomal cytochrome P-450b without the use of nonionic detergent.不使用非离子去污剂纯化大鼠肝脏微粒体细胞色素P-450b。
J Biochem Toxicol. 1988 Summer;3:131-45. doi: 10.1002/jbt.2570030207.
8
Kinetics of testosterone 6beta-hydroxylation in the reconstituted system with similar ratios of purified CYP3A4, NADPH-cytochrome p450 oxidoreductase and cytochrome B5 to human liver microsomes.在重组系统中，纯化的细胞色素P450 3A4、NADPH-细胞色素P450氧化还原酶和细胞色素B5与人类肝微粒体比例相似时睾酮6β-羟化反应的动力学。
Res Commun Mol Pathol Pharmacol. 2001 Jul;109(1-2):53-63.
9
Induction of liver microsomal cytochrome P450 in cynomolgus monkeys.食蟹猴肝脏微粒体细胞色素P450的诱导
Drug Metab Dispos. 1995 Jul;23(7):736-48.
10
Effect of mutations of ionic amino acids of cytochrome P450 1A2 on catalytic activities toward 7-ethoxycoumarin and methanol.细胞色素P450 1A2离子氨基酸突变对其催化7-乙氧基香豆素和甲醇活性的影响。
Biochemistry. 1993 Jun 1;32(21):5622-8. doi: 10.1021/bi00072a018.

引用本文的文献

1
Kinetics of carbon monoxide binding to phenobarbital-induced cytochrome P-450 from rat liver microsomes: a simple bimolecular process.一氧化碳与苯巴比妥诱导的大鼠肝微粒体细胞色素P-450结合的动力学：一个简单的双分子过程。
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4900-4. doi: 10.1073/pnas.82.15.4900.