Lee D M, Koren E, Singh S, Mok T
Biochem Biophys Res Commun. 1984 Sep 28;123(3):1149-56. doi: 10.1016/s0006-291x(84)80253-3.
Molecular forms of apolipoprotein B (ApoB) were studied in the rat intestinal chyle by SDS-polyacrylamide gel electrophoresis, immunoblotting and immunodiffusion. Time studies on intestinal chyle showed the presence of B-100 in all the samples analyzed within 3 hr after drawing. However, the analyses repeated on day 2 or day 3 revealed disappearance of B-100 and appearance of B-48. Addition of 3 mM EDTA, 10 mM diisopropylfluorophosphate, 5 mM chloroquine and 10 mM epsilon-amino caproic acid slowed down but could not prevent the disappearance of B-100. Chylomicrons isolated from chyle in the presence of preservatives immediately after drawing displayed B-100 as a major and B-48 as a minor ApoB form. However, repeatedly washed chylomicrons or those isolated from chyle 18-24 hr after drawing showed B-48 as the only ApoB present. These results suggest that rat intestine synthesizes B-100 which is quickly converted to smaller molecular form.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹和免疫扩散法研究了大鼠肠乳糜中载脂蛋白B（ApoB）的分子形式。对肠乳糜的时间研究表明，在采血后3小时内分析的所有样本中均存在B-100。然而，在第2天或第3天重复分析时，发现B-100消失，B-48出现。添加3 mM乙二胺四乙酸、10 mM二异丙基氟磷酸、5 mM氯喹和10 mMε-氨基己酸可减缓但不能阻止B-100的消失。采血后立即在防腐剂存在下从乳糜中分离的乳糜微粒显示，B-100是主要的ApoB形式，B-48是次要的ApoB形式。然而，反复洗涤的乳糜微粒或采血后18-24小时从乳糜中分离的乳糜微粒显示，B-48是唯一存在的ApoB。这些结果表明，大鼠肠道合成B-100，其可迅速转化为较小的分子形式。
