通过高效液相色谱法分离多氯联苯（Aroclor 1254）

Separation of polychlorinated biphenyls (Aroclor 1254) by high-performance liquid chromatography.

作者信息

Issaq H J, Klose J, Muschik G M

出版信息

J Chromatogr. 1984 Oct 19;302:159-66. doi: 10.1016/s0021-9673(01)89007-5.

Abstract

The separation of Aroclor 1254 on various reversed-phase columns was investigated. The results show that the cyano- and phenyl-bonded columns performed poorly, but C18 bonded columns gave better results. It was also found that the absorption wavelength at which the effluent is monitored has great effect on the detection of these isomers. The optimum conditions for the separation and detection are: C18, 5 micron; reversed-phase column, 20 cm X 4.6 mm I.D.; acetonitrile-water (60-40); flow-rate 1.5 ml/min; detection at 210 nm or 205 nm.

摘要

研究了Aroclor 1254在各种反相柱上的分离情况。结果表明，氰基键合柱和苯基键合柱的分离效果较差，但C18键合柱效果较好。还发现监测流出物的吸收波长对这些异构体的检测有很大影响。分离和检测的最佳条件为：C18，5微米；反相柱，20厘米×4.6毫米内径；乙腈-水（60-40）；流速1.5毫升/分钟；在210纳米或205纳米处检测。