嗜热栖热放线菌和嗜热假诺卡氏菌的生长及纤维素酶产生

Growth and cellulase production of Micromonospora chalcae and Pseudonocardia thermophila.

作者信息

Malfait M, Godden B, Penninckx M J

出版信息

Ann Microbiol (Paris). 1984 Jul-Aug;135B(1):79-89. doi: 10.1016/s0769-2609(84)80045-9.

Abstract

Beta(1,4)glucosidases and carboxymethylcellulases were demonstrated in both strains when using carboxymethylcellulose as a carbon source for growth. Beta(1,4)Glucosidases appeared mainly as cell-bound activities, whereas carboxymethylcellulases were evenly distributed between the incubation fluids and the cellular fractions. In both microorganisms, glucose appeared to repress biosynthesis of the enzymes, and cellobiose and carboxymethylcellulose acted as inducers of the cellulase complex.

摘要

当使用羧甲基纤维素作为生长的碳源时，在两种菌株中均证实存在β(1,4)葡萄糖苷酶和羧甲基纤维素酶。β(1,4)葡萄糖苷酶主要表现为细胞结合活性，而羧甲基纤维素酶则均匀分布于培养液和细胞组分之间。在这两种微生物中，葡萄糖似乎会抑制这些酶的生物合成，而纤维二糖和羧甲基纤维素则作为纤维素酶复合物的诱导剂。