一种链球菌细胞壁四杂聚糖和沙门氏菌特拉维夫脂多糖与聚合型McPC 870和MOPC 384小鼠IgA骨髓瘤蛋白的相互作用。

Interaction of a streptococcal cell wall tetraheteroglycan and Salmonella telaviv lipopolysaccharide with polymeric McPC 870 and MOPC 384 mouse IgA myeloma proteins.

作者信息

Allen P Z, Pazur J H

出版信息

Mol Immunol. 1984 Nov;21(11):1131-41. doi: 10.1016/0161-5890(84)90124-x.

Abstract

Interactions of polymeric McPC 870 and MOPC 384 mouse IgA myeloma proteins with a streptococcal cell wall tetraheteroglycan (T4-antigen), Salmonella tranoroa lipopolysaccharide (LPS), S. telaviv LPS and its lipid A free polysaccharide were examined by the quantitative precipitin method. Hapten inhibition of myeloma protein precipitation by T4-antigen or S. telaviv LPS indicates a complex anticarbohydrate specificity for 870 and 384 protein combining sites involving several sugars. While the combining site of 870 protein appears to bind alpha-D-mannopyranosyl, alpha-D-galactopyranosyl and alpha-D-glucopyranosyl residues, the combining site of 384 protein can accommodate both the alpha (1----2)-linked glucobiose (kojibiose) and alpha-D-galactopyranosyl but not the alpha-D-mannopyranosyl structure.

摘要

采用定量沉淀法检测了聚合型McPC 870和MOPC 384小鼠IgA骨髓瘤蛋白与链球菌细胞壁四杂聚糖（T4抗原）、肠炎沙门氏菌脂多糖（LPS）、特拉维夫沙门氏菌LPS及其无脂质A的多糖之间的相互作用。T4抗原或特拉维夫沙门氏菌LPS对半抗原抑制骨髓瘤蛋白沉淀的作用表明，870和384蛋白结合位点具有涉及多种糖类的复杂抗碳水化合物特异性。虽然870蛋白的结合位点似乎能结合α-D-甘露吡喃糖基、α-D-半乳吡喃糖基和α-D-葡吡喃糖基残基，但384蛋白的结合位点既能容纳α(1→2)连接的葡萄糖二糖（曲二糖），也能容纳α-D-半乳吡喃糖基，但不能容纳α-D-甘露吡喃糖基结构。