Hadlow W J, Jackson T A, Race R E
Am J Vet Res. 1984 Dec;45(12):2637-9.
Fetal (n = 21) and newborn (n = 7) Suffolk sheep were inoculated with scrapie virus isolated from other Suffolk sheep. Twenty fetuses, 76 to 109 days of gestational age, were inoculated IM in the neck through the uterine wall and were examined for virus 47 to 322 days later by mouse inoculation. Scrapie virus was not detected before 254 days of age; only traces of virus were detected in 3 of 7 lambs examined thereafter (2 at 254 days of age and 1 at 322 days of age). Virus was limited to the supra-pharyngeal, prescapular, and mesenteric lymph nodes. Seven lambs were inoculated into the palatine tonsils with scrapie virus as newborns (3 to 12 days old) and were examined for virus when they were 147 to 210 days old. Virus was not detected in the lymphoreticular tissues or terminal portion of ileum of any lamb. Failure to find scrapie virus in these lambs and in most lambs inoculated as fetuses might indicate few had became infected. However, if most lambs and fetuses had become infected, the long zero phase of the infection could have accounted for failure to find scrapie virus in many of them examined too soon after inoculation. The limited findings of this study indicate that efforts to demonstrate prenatal or neonatal transmission of scrapie by detecting virus are hampered by the slowness of its replication.
将来自其他萨福克羊的羊瘙痒病病毒接种到21只胎儿（胎龄76至109天）和7只新生萨福克羊体内。20只胎龄76至109天的胎儿通过子宫壁在颈部进行肌肉注射接种，并在接种47至322天后通过小鼠接种检测病毒。在254日龄之前未检测到羊瘙痒病病毒；在此之后检测的7只羔羊中有3只仅检测到微量病毒（2只在254日龄，1只在322日龄）。病毒局限于咽上、肩胛前和肠系膜淋巴结。7只新生羔羊（3至12日龄）通过腭扁桃体接种羊瘙痒病病毒，并在147至210日龄时检测病毒。在任何羔羊的淋巴网状组织或回肠末端均未检测到病毒。在这些羔羊以及大多数作为胎儿接种的羔羊中未发现羊瘙痒病病毒，这可能表明很少有羔羊被感染。然而，如果大多数羔羊和胎儿已被感染，那么感染的长时间零期可能是导致在接种后过早检测的许多羔羊中未发现羊瘙痒病病毒的原因。本研究的有限结果表明，通过检测病毒来证明羊瘙痒病的产前或新生儿传播的努力因病毒复制缓慢而受阻。
