Port J L, DeVoe I W, Archibald F S
Can J Microbiol. 1984 Dec;30(12):1453-7. doi: 10.1139/m84-232.
Group B Neisseria meningitidis (SD1C) was grown on defined medium supplemented with each of a variety of sulphur compounds as the sole source of sulphur. The organism grew on sulphate, sulphite, bisulphite, thiosulphate, dithionite, hydrosulphide, thiocyanate, L-cysteine, L-cystine, reduced glutathione, methionine, mercaptosuccinate, and lanthionine, but not on dithionate unless previously sulphur starved. Good growth was seen on concentrations of sulphate or thiosulphate as low as 10 microM. When pregrown on and subsequently starved for sulphate, the meningococcus showed enhanced transport capacity for this ion. Optimal conditions for assessing sulphur transport by active sulphur-limited cells were determined. The maximal sulphate uptake velocity was 9.3 nmol sulphate X mg protein-1 X min-1, and the apparent Km was 1.4 microM, far below human nasopharyngeal or serum sulphate levels.
B 群脑膜炎奈瑟菌（SD1C）在添加了多种硫化合物中的每一种作为唯一硫源的限定培养基上生长。该生物体能在硫酸盐、亚硫酸盐、亚硫酸氢盐、硫代硫酸盐、连二亚硫酸盐、氢硫化物、硫氰酸盐、L-半胱氨酸、L-胱氨酸、还原型谷胱甘肽、蛋氨酸、巯基琥珀酸盐和羊毛硫氨酸上生长，但除非先前处于硫饥饿状态，否则在连二硫酸盐上不能生长。在低至 10 μM 的硫酸盐或硫代硫酸盐浓度下可见良好生长。当在硫酸盐上预生长并随后使其处于硫饥饿状态时，脑膜炎球菌对该离子的转运能力增强。确定了评估活性硫限制细胞硫转运的最佳条件。最大硫酸盐摄取速度为 9.3 nmol 硫酸盐×mg 蛋白-1×min-1，表观 Km 为 1.4 μM，远低于人类鼻咽或血清中的硫酸盐水平。
