正常人黑质中的酪氨酸酶样活性。
Tyrosinase-like activity in normal human substantia nigra.
作者信息
Miranda M, Botti D, Bonfigli A, Ventura T, Arcadi A
出版信息
Gen Pharmacol. 1984;15(6):541-4. doi: 10.1016/0306-3623(84)90212-x.
PMID:6441736
Abstract
A tyrosinase-like activity was found in human substantia nigra by polyacrylamide gel electrophoresis of fractions prepared from homogenates of the substantia nigra. The enzyme activity was detected by staining the gels with L-3,4-dihydroxyphenylalanine, dopamine and 5,6-dihydroxyindole as substrates for tyrosinase (EC 1.14.18.1). A case of parkinsonism does not show the L-3,4-dihydroxyphenylalanine and dopamine oxidase activities.
摘要
通过对人类黑质匀浆制备的组分进行聚丙烯酰胺凝胶电泳，在人类黑质中发现了一种类似酪氨酸酶的活性。通过用L-3,4-二羟基苯丙氨酸、多巴胺和5,6-二羟基吲哚作为酪氨酸酶（EC 1.14.18.1）的底物对凝胶进行染色来检测酶活性。一例帕金森病患者未表现出L-3,4-二羟基苯丙氨酸和多巴胺氧化酶活性。
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