Dijkmans H, Daniel A M
Can J Biochem. 1981 Jan;59(1):16-21. doi: 10.1139/o81-003.
The binding of the chloroplast coupling factor CF to lipid vesicles was analyzed by gel filtration. CF can be bound to vesicles made of chloroplast lipids but not of lecithin. The presence in the vesicle walls of a proteolipid subunit of the hydrophobic component of the coupling factor increases the binding of CF. The apparent binding constant and the maximum protein/lipid ratio are calculated. The Ca2+-ATPase activity of bound CF is markedly lower than that of dissolved CF. It is confirmed that the proteolipid is a N,N'-dicyclohexylcarbodiimide sensitive proton channel. The binding of CF on proteolipid vesicles decreases their proton permeability.
通过凝胶过滤分析了叶绿体偶联因子CF与脂质小泡的结合情况。CF可与由叶绿体脂质制成的小泡结合，但不能与卵磷脂制成的小泡结合。偶联因子疏水成分的蛋白脂质亚基存在于小泡壁中会增加CF的结合。计算了表观结合常数和最大蛋白质/脂质比。结合态CF的Ca2+-ATP酶活性明显低于溶解态CF。证实该蛋白脂质是一种对N，N'-二环己基碳二亚胺敏感的质子通道。CF在蛋白脂质小泡上的结合降低了它们的质子渗透性。
